Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Escherichia coli | |
methotrexate | - |
Escherichia coli | |
Na+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00044 | - |
7,8-dihydrofolate | - |
Escherichia coli | |
0.00645 | - |
NADPH | - |
Escherichia coli | |
0.32 | - |
NADH | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17300 | 18300 | methotrexate-resistant mutant, equilibrium sedimentation | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli | - |
methotrexate-resistant mutant | - |
Escherichia coli MB 1428 / B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
methotrexate-resistant mutant: large-scale | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36 | - |
methotrexate resistant, 23°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADH + H+ | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NAD+ | - |
r | |
7,8-dihydrofolate + NADH + H+ | - |
Escherichia coli MB 1428 / B / ATCC 11303 | 5,6,7,8-tetrahydrofolate + NAD+ | - |
r | |
folate + NADPH | no activity | Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
folate + NADPH | no activity | Escherichia coli MB 1428 / B / ATCC 11303 | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
NADPH | per binding site | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
methotrexate-resistant cells, broad maximum | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.2 | 8.8 | half-maximal activities at pH 4.2 and pH 8.8, acetate buffer, Tris buffer | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH oxidized 5.1-times more rapidly than NADH | Escherichia coli | |
NADPH | NADPH oxidized 5.1-times more rapidly than NADH | Escherichia coli |