KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADH | reaction with DELTA1-piperidine 2-carboxylate | Bos taurus | |
0.24 | - |
S-Aminoethylcysteine ketimine | reaction with NADH | Bos taurus | |
0.26 | - |
NADH | reaction with DELTA1-piperidine 2-carboxylate | Bos taurus | |
0.33 | - |
DELTA1-Piperidine 2-carboxylate | reaction with DELTA1-piperidine-2-carboxylate | Bos taurus | |
1.17 | - |
Lanthionine ketimine | reaction with NADPH | Bos taurus | |
10 | - |
Cystathionine ketimine | reaction with NADPH | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Bos taurus |
100000 | - |
non-denaturing PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H + H+ | classical ping-pong mechanism | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | localized prevalently in cerebellum and cerebral cortices | Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.6 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, purified enzyme is stable for at least 1 month | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cystathionine ketimine + NADH | - |
Bos taurus | cyclothionine + NAD+ | - |
? | |
cystathionine ketimine + NADPH | - |
Bos taurus | cyclothionine + NADP+ | - |
? | |
DELTA1-piperideine 2-carboxylate + NADH | - |
Bos taurus | ? + NAD+ | - |
? | |
DELTA1-piperideine 2-carboxylate + NADPH | - |
Bos taurus | ? + NADP+ | - |
? | |
lanthionine ketimine + NADH | - |
Bos taurus | 1,4-thiomorpholine 3,5-dicarboxylic acid + NAD+ | - |
? | |
lanthionine ketimine + NADPH | - |
Bos taurus | 1,4-thiomorpholine 3,5-dicarboxylic acid + NADP+ | - |
? | |
S-aminoethylcysteine ketimine + NADH | - |
Bos taurus | 1,4-thiomorpholine 3-carboxylic acid + NAD+ | - |
? | |
S-aminoethylcysteine ketimine + NADPH | - |
Bos taurus | 1,4-thiomorpholine 3-carboxylic acid + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
reaction with lanthionine ketimine and NADPH | Bos taurus |
5 | - |
reaction with S-aminoethylcysteine ketimine and NADH, cystathionine ketimine and NADPH or 1-piperidine 2-carboxylate and NADPH | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | reduction rate for cystathionine ketimine, lanthionine ketimine and DELTA1-piperidine 2-carboxylate is higher with NADPH than with NADH. Reduction rate for S-aminoethylcysteine with NADH is higher than with NADPH | Bos taurus | |
NADPH | reduction rate for cystathionine ketimine, lanthionine ketimine and DELTA1-piperidine 2-carboxylate is higher with NADPH than with NADH. Reduction rate for S-aminoethylcysteine with NADH is higher than with NADPH | Bos taurus |