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Literature summary for 1.5.1.24 extracted from

  • Thompson, J.; Hill, K.K.; Smith, T.J.; Pikis, A.
    The gene CBO0515 from Clostridium botulinum strain Hall A encodes the rare enzyme N5-(carboxyethyl) ornithine synthase, EC 1.5.1.24 (2010), J. Bacteriol., 192, 1151-1155.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Clostridium botulinum

Protein Variants

Protein Variants Comment Organism
D114A severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
E13A severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
H92L severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
K71R severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
R15K severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum
W90S severe reduction (higher than 95%) in enzymatic activity Clostridium botulinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 pyruvate pH 7.5, temperature not specified in the publication, Vmax: 0.9 micromol/min/mg protein Clostridium botulinum
2.95
-
 NADPH pH 7.5, temperature not specified in the publication Clostridium botulinum
29.5
-
 L-ornithine pH 7.5, temperature not specified in the publication Clostridium botulinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35850
-
 calculated from cDNA Clostridium botulinum
36000
-
 SDS-PAGE Clostridium botulinum
125000
-
 gel filtration Clostridium botulinum

Organism

Organism UniProt Comment Textmining
Clostridium botulinum A5HZ59 UniProt
-

Purification (Commentary)

Purification (Comment) Organism
using anion-exchange and phosphocellulose P-11 chromatography Clostridium botulinum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.6
-
 pH 7.5, temperature not specified in the publication Clostridium botulinum

Storage Stability

Storage Stability Organism
-20°C, 20% glycerol, freezing and thawing results in loss of all enzymatic activity Clostridium botulinum
-20°C, 25% (NH4)2SO4, several months, no loss in activity Clostridium botulinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-acetylornithine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
D-lysine + pyruvate + NADPH + H+ enzyme also reacts with D-isomer Clostridium botulinum ? + NADP+ + H2O
-
 ?
D-ornithine + pyruvate + NADPH + H+ enzyme also reacts with D-isomer Clostridium botulinum N5-(L-1-carboxyethyl)-D-ornithine + NADP+ + H2O
-
 ?
L-lysine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
L-ornithine + 2-oxo-butanoic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
L-ornithine + 3-fluoropyruvic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
L-ornithine + 3-mercaptopyruvic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
L-ornithine + glyoxylic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
L-ornithine + methylglyoxal + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
L-ornithine + oxaloacetic acid + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
L-ornithine + pyruvate + NADPH + H+ highest reaction rate Clostridium botulinum N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
 ?
N2-methylornithine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
N6-hydroxy-L-lysine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
O-(2-aminoethyl)-L-serine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?
S-(2-aminoethyl)-L- cysteine + pyruvate + NADPH + H+ weak reaction rate Clostridium botulinum ? + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer in solution, gel filtration Clostridium botulinum

Synonyms

Synonyms Comment Organism
bCEOS
-
 Clostridium botulinum
CBO0515
-
 Clostridium botulinum
N5-(carboxyethyl) ornithine synthase
-
 Clostridium botulinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Clostridium botulinum