Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | slight activation at 1 mM | Pseudomonas putida | |
EDTA | slight activation at 1 mM | Pseudomonas putida | |
Sodium diphosphate | slight activation at 1 mM | Pseudomonas putida |
General Stability | Organism |
---|---|
dithiothreitol, 1 mM, protects during storage | Pseudomonas putida |
EDTA, 1 mM, protects during storage and against heat inactivation at 50°C | Pseudomonas putida |
NADP+, no protection during storage | Pseudomonas putida |
NADPH, protection during storage | Pseudomonas putida |
sodium diphosphate, 1 mM, protects during storage | Pseudomonas putida |
very unstable during purification | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight inhibition | Pseudomonas putida | |
Co2+ | almost total inhibition at 0.1 mM | Pseudomonas putida | |
Hg2+ | almost total inhibition at 0.1 mM | Pseudomonas putida | |
KCN | slight inhibition | Pseudomonas putida | |
Mg2+ | slight inhibition | Pseudomonas putida | |
Mn2+ | almost total inhibition at 0.1 mM | Pseudomonas putida | |
additional information | no effect: 0.1 mM L-cysteine, 0.1 mM iodoacetate | Pseudomonas putida | |
NADPH | substrate inhibition above 0.15 mM | Pseudomonas putida | |
NaF | - |
Pseudomonas putida | |
p-chloromercuribenzoate | almost total inhibition at 0.1 mM | Pseudomonas putida | |
Zn2+ | almost total inhibition at 0.1 mM | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NADPH | - |
Pseudomonas putida | |
0.23 | - |
DELTA1-piperideine-2-carboxylate | - |
Pseudomonas putida | |
0.28 | - |
diglutamate | - |
Pseudomonas putida | |
0.4 | - |
DELTA1-Pyrroline-5-carboxylate | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DELTA1-piperideine-2-carboxylate + NADPH | Pseudomonas putida | D-lysine metabolism | L-pipecolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.3 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
4°C, total loss of activity of the partially purified enzyme after 8 h, loss of activity can be prevented by adding low concentrations of EDTA, sodium diphosphate, dithiothreitol or L-pipecolic acid | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DELTA1-piperideine-2-carboxylate + NADPH | - |
Pseudomonas putida | L-pipecolate + NADP+ | - |
ir | |
DELTA1-piperideine-2-carboxylate + NADPH | D-lysine metabolism | Pseudomonas putida | L-pipecolate + NADP+ | - |
? | |
DELTA1-pyrroline-5-carboxylate + NADPH + H+ | very little activity | Pseudomonas putida | ? | - |
r | |
diglutamate + NADPH + H+ | very little activity | Pseudomonas putida | ? | - |
r | |
additional information | poor substrates: DELTA1-pyrroline-5-carboxylate, pyroglutamate | Pseudomonas putida | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.3 | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | poor coenzyme | Pseudomonas putida | |
NADPH | - |
Pseudomonas putida |