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Literature summary for 1.5.1.20 extracted from

  • Igari, S.; Ohtaki, A.; Yamanaka, Y.; Sato, Y.; Yohda, M.; Odaka, M.; Noguchi, K.; Yamada, K.
    Properties and crystal structure of methylenetetrahydrofolate reductase from Thermus thermophilus HB8 (2011), PLoS ONE, 6, e23716.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M sodium acetate buffer (NaOAc, pH 4.3,4.5), 1 M lithium chloride, 10% (w/v) polyethylene glycol 6000, 10-20% (v/v) glycerol, and 2-5% (v/v) dioxane, at 20°C Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
A222V the mutant shows enhanced instability upon loss of FAD Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
(6R)-5,10-methylenetetrahydrofolate at pH 7.0 and 50°C Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6R)-5,10-methylenetetrahydrofolate + NAD(P)H + H+ Thermus thermophilus
-
(6S)-5-methyltetrahydrofolate + NAD(P)+
-
r
(6R)-5,10-methylenetetrahydrofolate + NAD(P)H + H+ Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
(6S)-5-methyltetrahydrofolate + NAD(P)+
-
r

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SLG6
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SLG6
-
-

Purification (Commentary)

Purification (Comment) Organism
Toyopearl SuperQ-650M column chromatography and nickel affinity column chromatography Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6R)-5,10-methylenetetrahydrofolate + NAD(P)H + H+
-
Thermus thermophilus (6S)-5-methyltetrahydrofolate + NAD(P)+
-
r
(6R)-5,10-methylenetetrahydrofolate + NAD(P)H + H+
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 (6S)-5-methyltetrahydrofolate + NAD(P)+
-
r

Subunits

Subunits Comment Organism
heterodimer x-ray crystallography Thermus thermophilus

Synonyms

Synonyms Comment Organism
MTHFR
-
Thermus thermophilus
NADH:CH2-H4folate oxidoreductase
-
Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains one FAD prosthetic group bound per dimer. Km for FAD is 0.005 mM. The enzyme activity of the FAD-replete enzyme is approximately 50% compared to the normal purified enzyme Thermus thermophilus
NADH
-
Thermus thermophilus
NADPH
-
Thermus thermophilus