Cloned (Comment) | Organism |
---|---|
expression of chimeric mutant in an enzyme-deficient strain, overexpression of the plant enzyme from Arabidopsis thaliana in an enzyme-deficient strain can complement the mutant and results in 8fold increased accumulation of S-adenosyl-L-methionine, mechanism overview | Saccharomyces cerevisiae |
overexpression of the wild-type enzyme and the chimeric mutant in a Saccharomyces cerevisiae enzyme-deficient strain, complementation of the enzyme-deficient strain, 8fold increased accumulation of S-adenosyl-L-methionine in case of recombinant wild-type expression in yeast, mechanism overview | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a chimeric enzyme comprising the yeast N-terminal domain and the Arabidopsis thaliana C-terminal domain, recombinant expression in and complementation of an enzyme-deficient mutant strain with altered sensitivity to S-adenosyl-L-methionine and altered cofactor specifcity | Arabidopsis thaliana |
additional information | construction of a chimeric enzyme comprising the yeast N-terminal domain and the Arabidopsis thaliana C-terminal domain, recombinant expression in and complementation of an enzyme-deficient mutant strain with altered sensitivity to S-adenosyl-L-methionine and altered cofactor specifcity | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | substrate inhibition | Arabidopsis thaliana | |
5,10-methylenetetrahydrofolate | substrate inhibition | Saccharomyces cerevisiae | |
additional information | no inhibition by S-adenosyl-L-methionine, which is no feed-back regulator of the methyl group biosynthesis pathway in plants | Arabidopsis thaliana | |
S-adenosyl-L-methionine | feed-back regulation of the methyl group biosynthesis pathway in vivo, the chimeric mutant enzyme is insensitive to inhibition by S-adenosyl-L-methionine | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
NADH | recombinant wild-type enzyme, pH 7.2 | Arabidopsis thaliana | |
0.0028 | - |
NADH | recombinant chimeric mutant enzyme, pH 7.2 | Arabidopsis thaliana | |
0.0028 | - |
NADH | recombinant chimeric mutant enzyme, pH 7.2 | Saccharomyces cerevisiae | |
0.0073 | - |
NADPH | recombinant chimeric mutant enzyme, pH 7.2 | Arabidopsis thaliana | |
0.0073 | - |
NADPH | recombinant chimeric mutant enzyme, pH 7.2 | Saccharomyces cerevisiae | |
0.011 | - |
5,10-methylenetetrahydrofolate | recombinant enzyme, with NADPH, pH 7.2 | Saccharomyces cerevisiae | |
0.021 | - |
NADPH | recombinant enzyme, pH 7.2 | Saccharomyces cerevisiae | |
0.106 | - |
5,10-methylenetetrahydrofolate | recombinant chimeric mutant enzyme, with NADH, pH 7.2 | Arabidopsis thaliana | |
0.106 | - |
5,10-methylenetetrahydrofolate | recombinant chimeric mutant enzyme, with NADH, pH 7.2 | Saccharomyces cerevisiae | |
0.152 | - |
5,10-methylenetetrahydrofolate | recombinant chimeric mutant enzyme, with NADPH, pH 7.2 | Arabidopsis thaliana | |
0.152 | - |
5,10-methylenetetrahydrofolate | recombinant chimeric mutant enzyme, with NADPH, pH 7.2 | Saccharomyces cerevisiae | |
0.287 | - |
5,10-methylenetetrahydrofolate | recombinant wild-type enzyme, with NADH, pH 7.2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
gene met13 | - |
Saccharomyces cerevisiae | P53128 | gene met13 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NAD(P)H | NADH is the preferred cofactor of the plant enzyme | Arabidopsis thaliana | 5-methyltetrahydrofolate + NAD(P)+ | - |
r | |
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Arabidopsis thaliana | 5-methyltetrahydrofolate + NAD+ | - |
r | |
5,10-methylenetetrahydrofolate + NADH + H+ | - |
Saccharomyces cerevisiae | 5-methyltetrahydrofolate + NAD+ | - |
r | |
5,10-methylenetetrahydrofolate + NADPH | - |
Saccharomyces cerevisiae | 5-methyltetrahydrofolate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AtMTHFR-1 | - |
Arabidopsis thaliana |
methylenetetrahydrofolate reductase | - |
Arabidopsis thaliana |
methylenetetrahydrofolate reductase | - |
Saccharomyces cerevisiae |
MTHFR | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | wild-type shows no activity with NADH/NAD+ | Saccharomyces cerevisiae | |
NAD+ | only active with the chimeric mutant enzyme, not with the wild-type | Saccharomyces cerevisiae | |
NAD+ | recombinant wild-type and chimeric mutant enzymes | Arabidopsis thaliana | |
NADH | only active with the chimeric mutant enzyme, not with the wild-type | Saccharomyces cerevisiae | |
NADH | recombinant wild-type and chimeric mutant enzymes | Arabidopsis thaliana | |
NADP+ | - |
Saccharomyces cerevisiae | |
NADP+ | poor cofactor for the recombinant wild-type enzyme, but utilized by the chimeric mutant enzyme with activity equal to the activity with NAD+ | Arabidopsis thaliana | |
NADPH | dependent on | Saccharomyces cerevisiae | |
NADPH | poor cofactor for the recombinant wild-type enzyme, but utilized by the chimeric mutant enzyme with activity equal to the activity with NADH | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.455 | - |
5,10-methylenetetrahydrofolate | recombinant enzyme, with NADPH, pH 7.2 | Saccharomyces cerevisiae | |
0.873 | - |
5,10-methylenetetrahydrofolate | recombinant wild-type enzyme, with NADH, pH 7.2 | Arabidopsis thaliana |