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Literature summary for 1.5.1.20 extracted from
- Pentaglutamate derivatives of folate as substrates for rat liver tetrahydropteroylglutamate methyltransferase and 5,10-methylenetetrahydrofolate reductase (1975), Can. J. Biochem., 53, 1020-1027.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | inhibits at high concentrations | Rattus norvegicus |  |
| S-adenosylmethionine | in vivo feedback-inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.003 | - |
(+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Rattus norvegicus | |
| 0.023 | - |
5-methyltetrahydrofolate | - |
Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
adult male Sprague-Dawley rats, fed a Purina lab chow diet | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| more than 100fold partial purification | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity is greater with the monoglutamate substrate than with the pentaglutamate | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate + reduced acceptor | equilibrium lies far in favor of 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate formation, pentaglutamate form binds to the same enzyme site as monoglutamate form | Rattus norvegicus | (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate + oxidized acceptor | menadione as acceptor for 5,10-methylene-5,6,7,8-tetrahydropteroylpentaglutamate formation | ? | |
| 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: reduced acceptor is FADH2 | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
| 5,10-methylenetetrahydrofolate + reduced acceptor | 5,10-methylene-5,6,7,8-tetrahydropteroylmonoglutamate as substrate | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
| 5,10-methylenetetrahydrofolate + reduced acceptor | equilibrium lies far in favor of 5-methyltetrahydrofolate formation | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
| 5,10-methylenetetrahydrofolate + reduced acceptor | reverse reaction: menadione as electron acceptor | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
| 5,10-methylenetetrahydrofolate + reduced acceptor | forward reaction: NADH as reduced acceptor | Rattus norvegicus | 5-methyltetrahydrofolate + oxidized acceptor | product is (+)-5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate | ? | |
| 5-methyltetrahydrofolate + NAD+ | - |
Rattus norvegicus | 5,10-methylenetetrahydrofolate + NADH + H+ | - |
r | |
| additional information | reverse reaction: not NADP+ as acceptor | Rattus norvegicus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
5-methyltetrahydrofolate and 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate as substrates | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | pH 6: about 70% of activity maximum, pH 8: about 35% of activity maximum with 5-methyl-5,6,7,8-tetrahydropteroylmonoglutamate, about 65% of activity maximum with 5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein, oxidized acceptor in reverse reaction | Rattus norvegicus | |
| FADH2 | flavoprotein, reduced acceptor in forward reaction | Rattus norvegicus | |
| NADH | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | 0.051 | (+)-5-methyl-5,6,7,8-tetrahydropteroylpentaglutamate | - |
Rattus norvegicus | |
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