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Literature summary for 1.5.1.20 extracted from

  • Vanoni, M.A.; Lee, S.; Floss, H.G.; Matthews, R.G.
    Stereochemistry of reduction of methylenetetrahydrofolate to methyltetrahydrofolate catalyzed by pig liver methylenetetrahydrofolate reductase (1990), J. Am. Chem. Soc., 112, 3987-3992.
No PubMed abstract available
Search for more literature for 1.5.1.20

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H + H+ mechanism, acceptor is free FAD, stereochemistry Sus scrofa
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: reduced acceptor is FADH2 Sus scrofa 5-methyltetrahydrofolate + oxidized acceptor
-
 ?
5,10-methylenetetrahydrofolate + reduced acceptor forward reaction: NADPH as reduced acceptor causes irreversible reduction of the flavin coenzyme FAD Sus scrofa 5-methyltetrahydrofolate + oxidized acceptor
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD enzyme-bound FAD as coenzyme Sus scrofa
FAD flavoprotein, oxidized acceptor in reverse reaction Sus scrofa
FADH2 flavoprotein, reduced acceptor in forward reaction Sus scrofa
NADPH NADPH-dependent reduction of methylenetetrahydrofolate, NADPH irreversibly reduces the enzyme-bound flavin Sus scrofa