Cloned (Comment) | Organism |
---|---|
expression in Esherichia coli | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | inhibitory on NADH-dependent reaction | Arabidopsis thaliana | |
additional information | not inhibitory: NAD+ | Arabidopsis thaliana | |
NaCl | inhibitory on NADH-dependent reaction | Arabidopsis thaliana | |
NADP+ | - |
Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
NADPH | pH 7.8, 35°C | Arabidopsis thaliana | |
0.096 | - |
1-pyrroline-5-carboxylate | cofactor NADPH, pH 7.8, 35°C | Arabidopsis thaliana | |
0.21 | - |
NADH | pH 7.8, 35°C | Arabidopsis thaliana | |
0.915 | - |
1-pyrroline-5-carboxylate | cofactor NADH, pH 7.8, 35°C | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | stimulation of NADPH-dependent reaction, 70% stimulation at 100 mM | Arabidopsis thaliana | |
NaCl | stimulation of NADPH-dependent reaction, 70% stimulation at 100 mM | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
- |
Arabidopsis thaliana |
354000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | F4K884 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | cell suspension culture established from cotyledon-derived calli | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NADH + H+ | - |
Arabidopsis thaliana | L-proline + NAD+ | - |
? | |
1-pyrroline-5-carboxylate + NADPH + H+ | - |
Arabidopsis thaliana | L-proline + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 12 * 28000, SDS-PAGE | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
694 | - |
NADPH | pH 7.8, 35°C | Arabidopsis thaliana | |
3049 | - |
NADH | pH 7.8, 35°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
cofactor NADH | Arabidopsis thaliana |
8.4 | - |
cofactor NADPH | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arabidopsis thaliana | isoelectric focusing | - |
4.7 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.032 | - |
cofactor NADH, pH 7.8, 35°C | Arabidopsis thaliana | NADP+ | |
1.44 | - |
cofactor NADPH, pH 7.8, 35°C | Arabidopsis thaliana | NADP+ | |
200 | - |
cofactor NADH, pH 7.8, 35°C | Arabidopsis thaliana | NaCl | |
200 | - |
cofactor NADH, pH 7.8, 35°C | Arabidopsis thaliana | KCl |