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Literature summary for 1.5.1.2 extracted from

  • Singh, P.; Tiwari, A.; Singh, S.; Asthana, R.
    Proline biosynthesizing enzymes (glutamate 5-kinase and pyrroline-5-carboxylate reductase) from a model cyanobacterium for desiccation tolerance (2013), Physiol. Mol. Biol. Plants, 19, 521-528.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Anabaena sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28140
-
 x * 28140, calculated from SDS-PAGE Anabaena sp.

Organism

Organism UniProt Comment Textmining
Anabaena sp.
-
-
-
Anabaena sp. PCC 7120
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni+2-NTA His-Trap resin column chromatography, and gel filtration Anabaena sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Anabaena sp. L-proline + NAD(P)+
-
 ?
1-pyrroline-5-carboxylate + NAD(P)H + H+
-
 Anabaena sp. PCC 7120 L-proline + NAD(P)+
-
 ?

Subunits

Subunits Comment Organism
? x * 28140, calculated from SDS-PAGE Anabaena sp.

Synonyms

Synonyms Comment Organism
P5CR
-
 Anabaena sp.

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Anabaena sp.
NADPH
-
 Anabaena sp.