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Literature summary for 1.5.1.2 extracted from

  • Nocek, B.; Chang, C.; Li, H.; Lezondra, L.; Holzle, D.; Collart, F.; Joachimiak, A.
    Crystal structures of delta1-pyrroline-5-carboxylate reductase from human pathogens Neisseria meningitides and Streptococcus pyogenes (2005), J. Mol. Biol., 354, 91-106.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.2

Cloned(Commentary)

Cloned (Comment) Organism
cloned into a pMCSG7 vector, generating an expression clone of a fusion protein with an N-terminal His6-tag and a TEV protease recognition site for expression in Escherichia coli Neisseria meningitidis
cloned into a pMCSG7 vector, generating an expression clone of a fusion protein with an N-terminal His6-tag and a TEV protease recognition site for expression in Escherichia coli Streptococcus pyogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure is determined at 2.0 A resolution, in complex with NADP+ at 2.1 A resolution Neisseria meningitidis
crystal structure is determined at 2.15 A resolution in complex with NADP+, and at 2.20 A in complex with L-proline Streptococcus pyogenes

Inhibitors

Inhibitors Comment Organism Structure
L-proline L-proline inhibits the inverse reaction at a concentration of 10-20 mM Neisseria meningitidis
L-proline L-proline inhibits the inverse reaction at a concentration of 10-20 mM Streptococcus pyogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58800
-
 estimated by gel fitration Neisseria meningitidis
284600
-
 estimated by gel fitration Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-proline + NADP+ Neisseria meningitidis
-
 1-pyrroline-5-carboxylate + NADPH + H+
-
 r
L-proline + NADP+ Streptococcus pyogenes
-
 1-pyrroline-5-carboxylate + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
-
-
Streptococcus pyogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using a HiTrap Ni-NTA column Neisseria meningitidis
using a HiTrap Ni-NTA column Streptococcus pyogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-proline + NADP+
-
 Neisseria meningitidis 1-pyrroline-5-carboxylate + NADPH + H+
-
 r
L-proline + NADP+
-
 Streptococcus pyogenes 1-pyrroline-5-carboxylate + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
decamer
-
 Streptococcus pyogenes
dimer
-
 Neisseria meningitidis

Synonyms

Synonyms Comment Organism
DELTA1-pyrroline-5-carboxylate reductase
-
 Neisseria meningitidis
DELTA1-pyrroline-5-carboxylate reductase
-
 Streptococcus pyogenes
P5CR
-
 Neisseria meningitidis
P5CR
-
 Streptococcus pyogenes
pyrroline-5-carboxylate reductase
-
 Neisseria meningitidis
pyrroline-5-carboxylate reductase
-
 Streptococcus pyogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 activity assay Neisseria meningitidis
22
-
 activity assay Streptococcus pyogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Neisseria meningitidis
8
-
 activity assay Streptococcus pyogenes

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Neisseria meningitidis
NADPH
-
 Streptococcus pyogenes