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Literature summary for 1.5.1.2 extracted from
- Delta 1-Pyrroline-5-carboxylate reductase from Bakers yeast. Purification, properties and its application in the assays of L-delta 1-pyrroline-5-carboxylate and L-ornithine in tissue (1980), Biochim. Biophys. Acta, 613, 318-323.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes during purification | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | 90% inhibition at 0.012 mM | Saccharomyces cerevisiae |  |
| N-ethylmaleimide | 90% inhibition at 0.02 mM, inhibition can be prevented by NADH | Saccharomyces cerevisiae | |
| p-hydroxymercuribenzoate | 90% inhibition at 0.008 mM, inhibition can be prevented by addition of excess dithiothreitol | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
NADH | - |
Saccharomyces cerevisiae | |
| 0.056 | - |
NADPH | - |
Saccharomyces cerevisiae | |
| 0.08 | - |
DL-pyrroline-5-carboxylate | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 125000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
| 15 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 50% glycerol, 0.1 mM dithiothreitol, stable for 6 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-pyrroline-5-carboxylate + NAD(P)H + H+ | - |
Saccharomyces cerevisiae | L-proline + NAD(P)+ | - |
? | |
| 1-pyrroline-5-carboxylate + NADH + H+ | - |
Saccharomyces cerevisiae | L-proline + NAD+ | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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