Cloned (Comment) | Organism |
---|---|
gene nos, and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + 2-oxoglutarate + NADPH + H+ | Agrobacterium tumefaciens | - |
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | - |
ir | |
L-arginine + 2-oxoglutarate + NADPH + H+ | Agrobacterium tumefaciens C58 / ATCC 33970 | - |
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | A9CL08 | - |
- |
Agrobacterium tumefaciens C58 / ATCC 33970 | A9CL08 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + 2-oxoglutarate + NADPH + H+ | - |
Agrobacterium tumefaciens | N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | - |
ir | |
L-arginine + 2-oxoglutarate + NADPH + H+ | the reduction reaction is highly preferred | Agrobacterium tumefaciens | N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | - |
? | |
L-arginine + 2-oxoglutarate + NADPH + H+ | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | - |
ir | |
L-arginine + 2-oxoglutarate + NADPH + H+ | the reduction reaction is highly preferred | Agrobacterium tumefaciens C58 / ATCC 33970 | N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O | - |
? | |
additional information | analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+ | Agrobacterium tumefaciens | ? | - |
? | |
additional information | analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+ | Agrobacterium tumefaciens C58 / ATCC 33970 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Atu6015 | - |
Agrobacterium tumefaciens |
flavin-containing opine dehydrogenase | - |
Agrobacterium tumefaciens |
nopaline synthase | - |
Agrobacterium tumefaciens |
NOS | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Agrobacterium tumefaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Agrobacterium tumefaciens |