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Literature summary for 1.5.1.11 extracted from

  • Watanabe, S.; Sueda, R.; Fukumori, F.; Watanabe, Y.
    Characterization of flavin-containing opine dehydrogenase from bacteria (2015), PLoS ONE, 10, e0138434 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
gene ocs, DNA and amino acid sequence determination, analysis, and comparisons, genetic structure, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + pyruvate + NADH + H+ Agrobacterium tumefaciens
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 N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
 ?
additional information Agrobacterium tumefaciens octopine [N2-(1-D-carboxyethyl)-L-arginine] is synthesized by a NAD(P)H-dependent soluble dehydrogenase that catalyzes the reductive condensation of pyruvate with L-arginine. Although the reaction may be reversible in vitro, the frequent use of the term synthase rather than dehydrogenase has emphasized the importance of biosynthesis, but not degradation, and distinguishes it from the mollusk octopine dehydrogenase ?
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 ?

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + pyruvate + NADH + H+
-
 Agrobacterium tumefaciens N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
 ?
L-arginine + pyruvate + NADH + H+ the reduction reaction is highly preferred Agrobacterium tumefaciens N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
 ?
additional information octopine [N2-(1-D-carboxyethyl)-L-arginine] is synthesized by a NAD(P)H-dependent soluble dehydrogenase that catalyzes the reductive condensation of pyruvate with L-arginine. Although the reaction may be reversible in vitro, the frequent use of the term synthase rather than dehydrogenase has emphasized the importance of biosynthesis, but not degradation, and distinguishes it from the mollusk octopine dehydrogenase Agrobacterium tumefaciens ?
-
 ?
additional information the enzyme OCS utilizes not only L-arginine, but also other amino acids, to yield the corresponding members of the octopine family. Analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+ Agrobacterium tumefaciens ?
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 ?

Synonyms

Synonyms Comment Organism
flavin-containing opine dehydrogenase
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 Agrobacterium tumefaciens
OCS
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 Agrobacterium tumefaciens
Octopine synthase
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 Agrobacterium tumefaciens
pTi_008
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 Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Agrobacterium tumefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Agrobacterium tumefaciens

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Agrobacterium tumefaciens