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Literature summary for 1.5.1.11 extracted from

  • Walsh, P.J.
    Purification and characterization of two allozymic forms of octopine dehydrogenase from california populations of Metridium senile (1981), J. Comp. Physiol., 143, 213-222.
No PubMed abstract available
Search for more literature for 1.5.1.11

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxypyruvate inhibitor of octopine formation Metridium senile
glyoxylate inhibitor of octopine formation Metridium senile
homoarginine inhibitor of octopine formation Metridium senile
octopine product inhibition Metridium senile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Metridium senile

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34900
-
 1 * 34900, SDS-PAGE Metridium senile
40000
-
 gel filtration Metridium senile

Organism

Organism UniProt Comment Textmining
Metridium senile
-
 2 allozymic forms are distributed in a highly population-specific manner, enzyme variants: ss, ff, and sf
-

Purification (Commentary)

Purification (Comment) Organism
-
 Metridium senile

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45.5
-
-
 Metridium senile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Canavanine + pyruvate + NADH 57.6-92.6% of the activity with Arg, depending on enzyme variant Metridium senile N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
 ?
Homoarginine + pyruvate + NADH 58.6-70.8% of activity with Arg, depending on enzyme variant Metridium senile N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
 ?
L-Arg + 2-oxobutanoate + NADH 20.6-29.0% of activity with Arg Metridium senile N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
 ?
L-Arg + oxaloacetate + NADH at 6.8-14.4% of activity with L-Arg Metridium senile ?
-
 ?
L-Arg + pyruvate + NADH
-
 Metridium senile N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
 ?
L-Lys + pyruvate + NADH 87.2-92.6% of activity with Arg, depending on enzyme variant Metridium senile Lysopine + NAD+ + H2O
-
 ?
additional information no activity with 2-hydroxybutanoate Metridium senile ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 34900, SDS-PAGE Metridium senile

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Metridium senile