Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-hydroxymercuribenzoate | - |
Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
NADP+ | - |
Saccharomyces cerevisiae | |
0.92 | - |
saccharopine | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73000 | - |
density gradient centrifugation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH | - |
Saccharomyces cerevisiae | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
saccharopine formation | Saccharomyces cerevisiae |
10 | - |
saccharopine degradation | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ and NADP+ equally effective in 2-aminoadipate 6-semialdehyde formation | Saccharomyces cerevisiae | |
NADH | NADPH far more effective than NADH in saccaropine formation | Saccharomyces cerevisiae | |
NADP+ | NAD+ and NADP+ equally effective in 2-aminoadipate 6-semialdehyde formation | Saccharomyces cerevisiae | |
NADPH | far more effective than NADH in saccharopine formation | Saccharomyces cerevisiae |