Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme disruption mutant, strain is unable to use D-lysine or D-proline as a sole carbon source | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | involved in the catabolism of D-lysine and D-proline | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.33 | - |
reduction of DELTA1-pyrroline-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C | Pseudomonas putida |
0.67 | - |
reduction of DELTA1-piperidine-2-carboxylate, cosubstrate NADH, pH 8.0, 30°C | Pseudomonas putida |
1.4 | - |
oxidation of L-pipecolate, cosubstrate NADP+, pH 8.0, 30°C | Pseudomonas putida |
5.4 | - |
oxidation of L-proline, cosubstrate NADP+, pH 8.0, 30°C | Pseudomonas putida |
51 | - |
reduction of DELTA1-pyrroline-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C | Pseudomonas putida |
92 | - |
reduction of DELTA1-piperidine-2-carboxylate, cosubstrate NADPH, pH 8.0, 30°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DELTA1-piperidine-2-carboxylate + NAD(P)H | - |
Pseudomonas putida | L-pipecolic acid + NAD(P)+ | - |
r | |
DELTA1-pyrroline-2-carboxylate + NAD(P)H | at 55% of the rate with DELTA1-piperidine-2-carboxylate | Pseudomonas putida | L-proline + NAD(P)+ | - |
r | |
L-pipecolic acid + NAD(P)+ | at 26% of the rate with L-proline | Pseudomonas putida | DELTA1-piperidine-2-carboxylate + NAD(P)H | - |
r | |
L-proline + NAD(P)+ | - |
Pseudomonas putida | DELTA1-pyrroline-2-carboxylate + NAD(P)H + H+ | - |
r | |
additional information | involved in the catabolism of D-lysine and D-proline | Pseudomonas putida | ? | - |
? | |
additional information | reverse reactions oxidizing L-proline or L-pipecolic acid are catalyzed at much lower rates. Very poor substrates: trans-4-hydroxy-L-proline, L-thioproline | Pseudomonas putida | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas putida | |
NADH | reaction with NADH is less than 1% of that with NADPH | Pseudomonas putida | |
NADP+ | - |
Pseudomonas putida | |
NADPH | reaction with NADH is less than 1% of that with NADPH | Pseudomonas putida |