BRENDA - Enzyme Database
show all sequences of 1.4.9.2

Electron transfer reactions between aromatic amine dehydrogenase and azurin

Hyun, Y.L.; Davidson, V.L.; Biochemistry 34, 12249-12254 (1995)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
azurin
in 0.25 M potassium phosphate buffer
Alcaligenes faecalis
0.137
-
azurin
in 0.01 M potassium phosphate buffer
Alcaligenes faecalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
aromatic amines + acceptor + H2O
Alcaligenes faecalis
physiological acceptor: azurin, not amicyanin
? + reduced acceptor + NH3
-
-
?
aromatic amines + acceptor + H2O
Alcaligenes faecalis IFO 14479
physiological acceptor: azurin, not amicyanin
? + reduced acceptor + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Alcaligenes faecalis
-
-
-
Alcaligenes faecalis IFO 14479
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ArCH2NH2 + H2O + 2 azurin
-
396593
Alcaligenes faecalis
ArCHO + NH3 + 2 reduced azurin
-
-
-
-
ArCH2NH2 + H2O + 2 azurin
-
396593
Alcaligenes faecalis IFO 14479
ArCHO + NH3 + 2 reduced azurin
-
-
-
-
aromatic amines + acceptor + H2O
physiological acceptor: azurin, not amicyanin
396593
Alcaligenes faecalis
? + reduced acceptor + NH3
-
-
-
?
aromatic amines + acceptor + H2O
physiological acceptor: azurin, not amicyanin
396593
Alcaligenes faecalis IFO 14479
? + reduced acceptor + NH3
-
-
-
?
tyramine + acceptor + H2O
electron acceptor: azurin
396593
Alcaligenes faecalis
4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
-
-
?
tyramine + acceptor + H2O
electron acceptor: azurin
396593
Alcaligenes faecalis IFO 14479
4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
64
-
azurin
in 0.01 M potassium phosphate buffer
Alcaligenes faecalis
91
-
azurin
in 0.25 M potassium phosphate buffer
Alcaligenes faecalis
Cofactor
Cofactor
Commentary
Organism
Structure
tryptophan tryptophylquinone
-
Alcaligenes faecalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
tryptophan tryptophylquinone
-
Alcaligenes faecalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.044
-
azurin
in 0.25 M potassium phosphate buffer
Alcaligenes faecalis
0.137
-
azurin
in 0.01 M potassium phosphate buffer
Alcaligenes faecalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
aromatic amines + acceptor + H2O
Alcaligenes faecalis
physiological acceptor: azurin, not amicyanin
? + reduced acceptor + NH3
-
-
?
aromatic amines + acceptor + H2O
Alcaligenes faecalis IFO 14479
physiological acceptor: azurin, not amicyanin
? + reduced acceptor + NH3
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ArCH2NH2 + H2O + 2 azurin
-
396593
Alcaligenes faecalis
ArCHO + NH3 + 2 reduced azurin
-
-
-
-
ArCH2NH2 + H2O + 2 azurin
-
396593
Alcaligenes faecalis IFO 14479
ArCHO + NH3 + 2 reduced azurin
-
-
-
-
aromatic amines + acceptor + H2O
physiological acceptor: azurin, not amicyanin
396593
Alcaligenes faecalis
? + reduced acceptor + NH3
-
-
-
?
aromatic amines + acceptor + H2O
physiological acceptor: azurin, not amicyanin
396593
Alcaligenes faecalis IFO 14479
? + reduced acceptor + NH3
-
-
-
?
tyramine + acceptor + H2O
electron acceptor: azurin
396593
Alcaligenes faecalis
4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
-
-
?
tyramine + acceptor + H2O
electron acceptor: azurin
396593
Alcaligenes faecalis IFO 14479
4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
64
-
azurin
in 0.01 M potassium phosphate buffer
Alcaligenes faecalis
91
-
azurin
in 0.25 M potassium phosphate buffer
Alcaligenes faecalis
Other publictions for EC 1.4.9.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
685206
Hothi
Catalysis by the isolated tryp ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Biochemistry
47
183-194
2008
-
-
-
-
-
-
1
-
-
-
-
-
-
10
-
-
1
-
-
1
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685188
Hothi
Isotope effects reveal that pa ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Biochemistry
46
9250-9259
2007
-
-
-
1
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
1
-
-
4
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687589
Roujeinikova
New insights into the reductiv ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
J. Biol. Chem.
282
23766-23777
2007
-
-
-
-
-
-
-
-
-
-
2
-
-
9
-
-
1
-
-
-
-
-
8
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672092
Sukumar
Crystal structure of an electr ...
Alcaligenes faecalis
Biochemistry
45
13500-13510
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673559
Hothi
Tryptophan tryptophylquinone c ...
Alcaligenes faecalis
FEBS J.
273
430
2006
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
1
-
-
1
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
674733
Roujeinikova
Atomic level insight into the ...
Alcaligenes faecalis
J. Biol. Chem.
281
40264-40272
2006
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677123
Massgrau
Atomic description of an enzym ...
Alcaligenes faecalis
Science
312
237-241
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655062
Kondo
Purification and characterizat ...
Achromobacter xylosoxidans, Achromobacter xylosoxidans IFO13495
Biosci. Biotechnol. Biochem.
68
1921-1928
2004
-
-
-
-
-
-
15
4
-
-
3
2
-
3
-
-
1
1
-
1
1
1
23
1
1
1
3
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
15
-
4
-
-
3
2
-
-
-
1
-
1
1
1
23
1
1
1
3
-
1
-
1
-
-
-
-
-
-
-
396596
Chistoserdov
Cloning, sequencing and mutage ...
Achromobacter xylosoxidans, Alcaligenes faecalis
Microbiology
147
2195-2202
2001
-
-
-
-
-
-
-
-
1
-
2
-
-
5
-
-
-
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396595
Hyun
Gated and ungated electron tra ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
J. Biol. Chem.
274
29081-29086
1999
-
-
-
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715340
Zhu
Localization of periplasmic re ...
Alcaligenes faecalis
J. Bacteriol.
181
6540-6542
1999
-
-
-
1
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714515
Adachi
Characterization of quinohemop ...
Pseudomonas putida, Pseudomonas putida IFO 15366
Biosci. Biotechnol. Biochem.
62
469-478
1998
-
-
-
1
-
-
4
-
-
-
4
-
-
2
-
-
-
-
-
-
1
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
4
-
-
-
-
4
-
-
-
-
-
-
-
1
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396593
Hyun
Electron transfer reactions be ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Biochemistry
34
12249-12254
1995
-
-
-
-
-
-
-
2
-
-
-
2
-
10
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
396594
Hyun
Unusually large isotope effect ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Biochim. Biophys. Acta
1251
198-200
1995
-
-
-
-
-
-
-
2
-
-
-
-
-
9
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
715426
Edwards
Spectroscopic evidence for a c ...
Alcaligenes faecalis
J. Biol. Chem.
270
4293-4298
1995
-
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396592
Govindaraj
Aromatic amine dehydrogenase, ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
J. Bacteriol.
176
2922-2929
1994
-
-
-
-
-
1
8
3
-
-
4
-
-
9
-
-
1
-
-
-
2
-
6
1
-
-
1
-
-
-
-
1
3
-
-
-
-
-
1
-
-
1
-
8
3
3
-
-
4
-
-
-
-
1
-
-
2
-
6
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288289
Cuskey
Initial catabolism of aromatic ...
Pseudomonas aeruginosa
J. Bacteriol.
169
2398-2404
1987
-
-
1
-
-
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396589
Nozaki
Aromatic amine dehydrogenase f ...
Alcaligenes faecalis, Alcaligenes faecalis IFO 14479
Methods Enzymol.
142
650-655
1987
-
-
-
1
-
-
3
-
-
-
3
-
-
10
-
-
1
-
-
-
1
-
10
1
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
3
-
-
-
-
3
-
-
-
-
1
-
-
1
-
10
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
396591
Iwaki
Crystallization and properties ...
Pseudomonas sp.
Arch. Biochem. Biophys.
220
253-262
1983
-
-
-
1
-
-
6
4
-
2
3
-
-
5
-
-
1
-
-
-
1
-
10
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
-
-
6
-
4
-
2
3
-
-
-
-
1
-
-
1
-
10
1
-
-
-
-
2
1
-
-
-
-
-
-
-
-