Literature summary for 1.4.9.1 extracted from

Arias, S.; Olivera, E.R.; Arcos, M.; Naharro, G.; Luengo, J.M.
Genetic analyses and molecular characterization of the pathways involved in the conversion of 2-phenylethylamine and 2-phenylethanol into phenylacetic acid in Pseudomonas putida U (2008), Environ. Microbiol., 10, 413-432.

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Cloned(Commentary)

Cloned (Comment)	Organism
Expression in Escherichia coli, the production of knock out-mutants unable to catabolize 2-phenylethylamine is carried out by mutagenesis.	Pseudomonas putida

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	enzyme contains two heme groups	Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
8579	-	calculated gamma-subunit	Pseudomonas putida
39360	-	calculated for beta-subunit, containing a 26 aa sequence which seems to correspond to a signal peptide that might be also involved in the correct translocation from the bacterial cytoplasm to the periplasmic space.	Pseudomonas putida
54230	-	calculated for alpha-subunit, existence in this protein of two consensus sequences (CXXCH) identifying as binding pockets for the two haem groups	Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2-phenylethylamine + 2 H2O + 2 acceptor	Pseudomonas putida	primary amine	2-phenylacetic acid + NH3 + 2 reduced acceptor	-	?
additional information	Pseudomonas putida	an essential enzyme for the aerobic degradation of many primary amines even though they have quite different chemical structures (aromatic or aliphatic)	?	-	?
additional information	Pseudomonas putida	when different QHNDH mutants (peaA, peaC and peaD) are transformed with a genetic construction containing the peaABCD cluster, all the recombinant strains efficiently catabolized 2-phenylethylamine as well as other primary amines like propyl-, butyl- and pentylamine.	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas putida	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cell culture	-	Pseudomonas putida	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-phenylethylamine + 2 H2O + 2 acceptor	primary amine	Pseudomonas putida	2-phenylacetic acid + NH3 + 2 reduced acceptor	-	?
additional information	an essential enzyme for the aerobic degradation of many primary amines even though they have quite different chemical structures (aromatic or aliphatic)	Pseudomonas putida	?	-	?
additional information	when different QHNDH mutants (peaA, peaC and peaD) are transformed with a genetic construction containing the peaABCD cluster, all the recombinant strains efficiently catabolized 2-phenylethylamine as well as other primary amines like propyl-, butyl- and pentylamine.	Pseudomonas putida	?	-	?

Subunits

Subunits	Comment	Organism
heterotrimer	determinated by in silico-experiments, beta-subunit with 349 aa, gamma-subunit with 79 aa and an alpha-subunit with 494 aa	Pseudomonas putida
More	protein PeaB, although not interacting with the active QHNDH along the catalytic reaction, is needed for the correct functionality of this enzyme and for the post-translational modification of the gamma-subunit before its translocation to the periplasmic space.	Pseudomonas putida

Synonyms

Synonyms	Comment	Organism
amine dehydrogenase	-	Pseudomonas putida
QH-AmDH	-	Pseudomonas putida
QHNDH	-	Pseudomonas putida
quinohaemoprotein amine dehydrogenase	-	Pseudomonas putida

Cofactor

Cofactor	Comment	Organism	Structure
cysteine tryptophylquinone	This enzyme contains an unusual redox cofactor (CTQ) and two hemes acting as redox active groups	Pseudomonas putida

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Release 2023.1 (January 2023)