Literature summary for 1.4.7.1 extracted from

Tripathy, J.N.; Hirasawa, M.; Sutton, R.B.; Dasgupta, A.; Vaidyanathan, N.; Zabet-Moghaddam, M.; Florencio, F.J.; Srivastava, A.P.; Knaff, D.B.
A loop unique to ferredoxin-dependent glutamate synthases is not absolutely essential for ferredoxin-dependent catalytic activity (2015), Photosynth. Res., 123, 129-139 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Synechocystis sp. PCC 6803

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0006	-	reduced ferredoxin	wild type enzyme, at pH 8.0, temperature not specified in the publication	Synechocystis sp. PCC 6803
0.04	-	2-oxoglutarate	wild type enzyme, at pH 8.0, temperature not specified in the publication	Synechocystis sp. PCC 6803
1.3	-	L-glutamine	wild type enzyme, at pH 8.0, temperature not specified in the publication	Synechocystis sp. PCC 6803

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	3 mol iron per mol enzyme	Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+	Synechocystis sp. PCC 6803	-	L-glutamate + oxidized ferredoxin	-	?

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp. PCC 6803	P55038	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, Sepharose column chromatography, and Superdex 200 gel filtration	Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+	-	Synechocystis sp. PCC 6803	L-glutamate + oxidized ferredoxin	-	?

Synonyms

Synonyms	Comment	Organism
ferredoxin-dependent glutamate synthase	-	Synechocystis sp. PCC 6803
glutamine:oxoglutarate amidotransferase	-	Synechocystis sp. PCC 6803
GOGAT	-	Synechocystis sp. PCC 6803

Cofactor

Cofactor	Comment	Organism	Structure
Ferredoxin	-	Synechocystis sp. PCC 6803
FMN	-	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)