Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | up to 50% increase in activity | Hydrogenobacter thermophilus | |
malate | up to 50% increase in activity | Hydrogenobacter thermophilus | |
oxaloacetate | up to 50% increase in activity | Hydrogenobacter thermophilus | |
succinate | up to 50% increase in activity | Hydrogenobacter thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
reduced bacterial ferredoxin 1 | in 20 mM NaPO4 (pH 7.2), at 70°C | Hydrogenobacter thermophilus | |
0.28 | - |
2-oxoglutarate | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
0.28 | - |
2-oxoglutarate | in 20 mM NaPO4 (pH 7.2), 10 mM L-glutamine, 5 mM methylviologen, 5 mM dithionite, at 70°C | Hydrogenobacter thermophilus | |
1.1 | - |
L-glutamine | pH 8.0, 70°C | Hydrogenobacter thermophilus | |
1.1 | - |
L-glutamine | in 20 mM NaPO4 (pH 7.2), 1 mM 2-oxoglutarate, 5 mM methylviologen, 5 mM dithionite, at 70°C | Hydrogenobacter thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
gel filtration | Hydrogenobacter thermophilus |
151000 | - |
SDS-PAGE | Hydrogenobacter thermophilus |
151000 | - |
1 * 151000, SDS-PAGE | Hydrogenobacter thermophilus |
168000 | - |
calculated from amino acid sequence | Hydrogenobacter thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hydrogenobacter thermophilus | - |
- |
- |
Hydrogenobacter thermophilus | A1IJT7 | - |
- |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | - |
- |
- |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | A1IJT7 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, hydroxyapatite column chromatography, butyl-Toyopearl column chromatography, MonoQ column chromatography, and DEAE-Toyopearl column chromatography | Hydrogenobacter thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0078 | - |
crude extract, at 70°C | Hydrogenobacter thermophilus |
0.6 | - |
pH 8.0, 70°C | Hydrogenobacter thermophilus |
1.5 | - |
after 191fold purification, at 70°C | Hydrogenobacter thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + reduced bacterial ferredoxin 1 | - |
Hydrogenobacter thermophilus | L-glutamate + oxidized bacterial ferredoxin 1 | - |
? | |
L-glutamine + 2-oxoglutarate + reduced bacterial ferredoxin 1 | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | L-glutamate + oxidized bacterial ferredoxin 1 | - |
? | |
L-glutamine + 2-oxoglutarate + reduced bacterial ferredoxin 2 | - |
Hydrogenobacter thermophilus | L-glutamate + oxidized bacterial ferredoxin 2 | - |
? | |
L-glutamine + 2-oxoglutarate + reduced bacterial ferredoxin 2 | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | L-glutamate + oxidized bacterial ferredoxin 2 | - |
? | |
L-glutamine + 2-oxoglutarate + reduced methylviologen | - |
Hydrogenobacter thermophilus | L-glutamate + oxidized methylviologen | - |
? | |
L-glutamine + 2-oxoglutarate + reduced methylviologen | - |
Hydrogenobacter thermophilus TK-6 / IAM 12695 | L-glutamate + oxidized methylviologen | - |
? | |
additional information | does not react with plant ferredoxin 3 | Hydrogenobacter thermophilus | ? | - |
? | |
additional information | does not react with plant ferredoxin 3 | Hydrogenobacter thermophilus TK-6 / IAM 12695 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 151000, SDS-PAGE | Hydrogenobacter thermophilus |
Synonyms | Comment | Organism |
---|---|---|
Fd-GOGAT | - |
Hydrogenobacter thermophilus |
ferredoxin-dependent glutamate synthase | exhibits also glutaminase activity and does not react with a plant-type ferredoxin (Fd3) containing a [2Fe-2S] cluster, but does react with bacterial ferredoxins (Fd1 and Fd2) containing [4Fe-4S] clusters | Hydrogenobacter thermophilus |
glutamine:2-oxoglutarate amidotransferase | - |
Hydrogenobacter thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | - |
Hydrogenobacter thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | 7.4 | at 70°C | Hydrogenobacter thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | enzyme does not react with plant-type ferredoxin but with a bacterial-type ferredoxin containing [4Fe-4S] clusters | Hydrogenobacter thermophilus | |
FMN | 0.9 mol per mol of protein | Hydrogenobacter thermophilus |