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Literature summary for 1.4.7.1 extracted from
- Purification and molecular properties of ferredoxin-glutamate synthase from Chlamydomonas reinhardtii (1984), Planta, 162, 180-187.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-diazo-5-oxo-norleucine | 5 mM, complete inhibition | Chlamydomonas reinhardtii |  |
| azaserine | 0.5 mM, complete inhibition | Chlamydomonas reinhardtii | |
| Bromocresol green | 1 mM, 89% inhibition | Chlamydomonas reinhardtii | |
| Flavianate | - |
Chlamydomonas reinhardtii | |
| N-ethylmaleimide | 0.05 mM, 94% inhibition | Chlamydomonas reinhardtii | |
| p-hydroxymercuribenzoate | - |
Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
reduced ferredoxin | - |
Chlamydomonas reinhardtii | |
| 0.7 | - |
L-glutamine | - |
Chlamydomonas reinhardtii | |
| 1 | - |
2-oxoglutarate | - |
Chlamydomonas reinhardtii | |
| 4.7 | - |
methyl viologen | - |
Chlamydomonas reinhardtii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 144000 | - |
sucrose density gradient centrifugation | Chlamydomonas reinhardtii |
| 151000 | - |
1 * 151000, SDS-PAGE | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + reduced ferredoxin | Chlamydomonas reinhardtii | highly specific for glutamine and 2-oxoglutarate | L-glutamate + oxidized ferredoxin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| protamine sulfate, DEAE-Sephacel, hydroxylapatite, ferredoxin-Sepharose | Chlamydomonas reinhardtii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Chlamydomonas reinhardtii | - |
| root | - |
Chlamydomonas reinhardtii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.4 | - |
- |
Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamine + 2-oxoglutarate + reduced ferredoxin | highly specific for glutamine and 2-oxoglutarate | Chlamydomonas reinhardtii | L-glutamate + oxidized ferredoxin | - |
? | |
| L-glutamine + 2-oxoglutarate + reduced ferredoxin + H+ | - |
Chlamydomonas reinhardtii | L-glutamate + oxidized ferredoxin | - |
r | |
| L-glutamine + 2-oxoglutarate + reduced methylviologen | artificial elctron donor | Chlamydomonas reinhardtii | L-glutamate + oxidized methylviologen | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 151000, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Chlamydomonas reinhardtii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Chlamydomonas reinhardtii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
full activity after 10 min, 50% activity remains after heating at 55°C for 10 min, complete loss of activity at 60°C | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Chlamydomonas reinhardtii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavin | enzyme may contain a flavin prosthetic group | Chlamydomonas reinhardtii | |
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