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Literature summary for 1.4.5.1 extracted from
- Purification and properties of D-amino acid dehydrogenase, an inducible membrane-bound iron-sulfur flavoenzyme from Escherichia coli B (1980), J. Biol. Chem., 255, 4487-4494.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| menaquinone | - |
Escherichia coli B |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
oxidized 2,6-dichlorophenolindophenol | coenzyme Q mediated | Escherichia coli B | |
| 0.63 | - |
oxidized 2,6-dichlorophenolindophenol | with D-alanine | Escherichia coli B | |
| 2 | - |
D-alanine | with ferricyanide | Escherichia coli B | |
| 3 | - |
D-alanine | with 2,6-dichlorophenolindophenol | Escherichia coli B | |
| 5.3 | - |
D-alanine | with coenzyme Q | Escherichia coli B | |
| 6 | - |
D-phenylalanine | with 2,6-dichlorophenolindophenol | Escherichia coli B | |
| 6.4 | - |
D-alanine | with 2,6-dichlorophenolindophenol, coenzyme Q mediated | Escherichia coli B | |
| 11 | - |
D-methionine | with 2,6-dichlorophenolindophenol | Escherichia coli B | |
| 12 | - |
D-2-amino-n-butyrate | with 2,6-dichlorophenolindophenol | Escherichia coli B | |
| 14 | - |
D-glutamine | with 2,6-dichlorophenolindophenol | Escherichia coli B | |
| 19 | - |
D-serine | with 2,6-dichlorophenolindophenol | Escherichia coli B | |
| 40 | - |
D-proline | with 2,6-dichlorophenolindophenol | Escherichia coli B | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | associated | Escherichia coli B | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | iron-sulfur flavoprotein | Escherichia coli B | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
x * 45000 + x * 55000, SDS-PAGE | Escherichia coli B |
| 55000 | - |
x * 45000 + x * 55000, SDS-PAGE | Escherichia coli B |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + oxidized acceptor | Escherichia coli B | the enzyme couples D-alanine oxidation to solute active transport in Escherichia coli B cytoplasmic membrane vesicles | pyruvate + NH3 + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli B | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli B |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Escherichia coli B |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.1 M potassium phosphate, pH 7.5, 0.02% Triton X-100, more than 1 mg protein/ml, stable for at least 3 months | Escherichia coli B |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-2-amino-n-butyrate + oxidized 2,6-dichlorophenolindophenol + H2O | 89% of the activity compared to D-alanine | Escherichia coli B | 2-oxobutyrate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-alanine + oxidized 2,6-dichloroindophenol + H2O | - |
Escherichia coli B | pyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-alanine + oxidized 2,6-dichlorophenolindophenol + H2O | r | Escherichia coli B | pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol | - |
? | |
| D-alanine + oxidized acceptor | the enzyme couples D-alanine oxidation to solute active transport in Escherichia coli B cytoplasmic membrane vesicles | Escherichia coli B | pyruvate + NH3 + reduced acceptor | - |
? | |
| D-alanine + oxidized coenzyme Q1 | no activity with menaquinone or adriamycin | Escherichia coli B | pyruvate + NH3 + reduced coenzyme Q1 | - |
? | |
| D-glutamine + oxidized 2,6-dichloroindophenol + H2O | - |
Escherichia coli B | 4-carbamoyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-methionine + oxidized 2,6-dichloroindophenol + H2O | 97% of the activity compared to D-alanine | Escherichia coli B | 4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-phenylalanine + oxidized 2,6-dichloroindophenol + H2O | as active as D-alanine | Escherichia coli B | phenylpyruvate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-proline + oxidized 2,6-dichloroindophenol | 28% of the activity compared to D-alanine | Escherichia coli B | DELTA1-pyrroline-2-carboxylate + reduced 2,6-dichloroindophenol | - |
? | |
| D-serine + oxidized 2,6-dichloroindophenol + H2O | 29% of the activity compared to D-alanine | Escherichia coli B | 3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
| D-valine + oxidized 2,6-dichloroindophenol + H2O | 11% of the activity compared to D-alanine | Escherichia coli B | 2-oxoisopentanoate + NH3 + reduced 2,6-dichloroindophenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000 + x * 55000, SDS-PAGE | Escherichia coli B |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.17 | - |
D-glutamine | - |
Escherichia coli B | |
| 5.33 | - |
D-serine | - |
Escherichia coli B | |
| 8.33 | - |
D-proline | - |
Escherichia coli B | |
| 14.7 | - |
D-phenylalanine | - |
Escherichia coli B | |
| 15.5 | - |
D-alanine | - |
Escherichia coli B | |
| 15.7 | - |
D-2-amino butyrate | - |
Escherichia coli B | |
| 16 | - |
D-methionine | - |
Escherichia coli B | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | iron-sulfur flavoprotein | Escherichia coli B | |
| ubiquinone | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol fold | Escherichia coli B | |
| ubiquinone-0 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 16fold | Escherichia coli B | |
| ubiquinone-1 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 25fold | Escherichia coli B | |
| ubiquinone-2 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 26fold | Escherichia coli B | |
| ubiquinone-3 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 21fold | Escherichia coli B | |
| ubiquinone-4 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 3fold | Escherichia coli B | |
| ubiquinone-5 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 2fold | Escherichia coli B | |
| ubiquinone-6 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 2fold | Escherichia coli B | |
| ubiquinone-7 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 2fold | Escherichia coli B | |
| ubiquinone-8 | 0.2 mM quinone stimulates reaction with D-alanine and oxidized 2,6-dichloroindophenol 2fold | Escherichia coli B | |
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