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Literature summary for 1.4.4.2 extracted from
- Proteins of the glycine decarboxylase complex in the hydrogenosome of Trichomonas vaginalis (2006), Eukaryot. Cell, 5, 2062-2071.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET-29B vector for expression in Escherichia coli BL21DE3 cells, into master-neo(HA)2 plasmid for transfection of Trichomonas vaginalis | Trichomonas vaginalis |
| pET-H1, pET-H2, and pET-L plasmids expressed in Escherichia coli BL21(DE3) cells | Trichomonas vaginalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
rTvH1 protein | the apparent Km-value of rTvL for binding rTvH1 | Trichomonas vaginalis | |
| 0.0026 | - |
H1 protein | L protein | Trichomonas vaginalis | |
| 0.0037 | - |
rTvH2 protein | the apparent Km-value of rTvL for binding rTvH2 | Trichomonas vaginalis | |
| 0.0037 | - |
H2 protein | L protein | Trichomonas vaginalis | |
| 0.043 | - |
Lipoamide | the apparent Km-value of rTvL for binding the free lipoamide | Trichomonas vaginalis |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| hydrogenosome | - |
Trichomonas vaginalis | 42566 | - |
| hydrogenosome | H1, H2, and L protein. H and L proteins do not form a stable complex in the hydrogenosomes | Trichomonas vaginalis | 42566 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13900 | - |
predicted value TvH1 protein | Trichomonas vaginalis |
| 14000 | - |
recombinant TvH1 protein, analysis of the native mass of TvH1 protein by gel filtration, SDS-PAGE and Western blot | Trichomonas vaginalis |
| 14800 | - |
predicted value TvH2 protein | Trichomonas vaginalis |
| 15000 | - |
recombinant TvH2 protein, analysis of the native mass of TvH2 protein by gel filtration, SDS-PAGE and Western blot | Trichomonas vaginalis |
| 51800 | - |
predicted value TvL protein | Trichomonas vaginalis |
| 52000 | - |
recombinant TvL protein, analysis of the native mass of TvL protein by gel filtration, SDS-PAGE and Western blot | Trichomonas vaginalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycine + H-protein-lipoyllysine | Trichomonas vaginalis | - |
H-protein-S-aminomethyldihydrolipoyllysine + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichomonas vaginalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| H1, H2, and L protein purified to homogeneity, more than 95% pure | Trichomonas vaginalis |
| using a nickel-nitrilotriacetic acid-agarose column | Trichomonas vaginalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycine + H-protein-lipoyllysine | - |
Trichomonas vaginalis | H-protein-S-aminomethyldihydrolipoyllysine + CO2 | - |
? | |
| glycine + lipoamide | - |
Trichomonas vaginalis | ? | - |
? | |
| glycine + rTvH1 protein | - |
Trichomonas vaginalis | ? | - |
? | |
| glycine + rTvH2 protein | - |
Trichomonas vaginalis | ? | - |
? | |
| H1 protein | substrate for L protein | Trichomonas vaginalis | ? | - |
? | |
| H2 protein | substrate for L protein | Trichomonas vaginalis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 52000, SDS-PAGE, L protein | Trichomonas vaginalis |
| monomer | 1 * 14000, SDS-PAGE, H1 protein, 1 * 15000, SDS-PAGE, H2 protein | Trichomonas vaginalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycine cleavage H protein | - |
Trichomonas vaginalis |
| glycine decarboxylase complex | - |
Trichomonas vaginalis |
| glycine dehydrogenase | - |
Trichomonas vaginalis |
| H1 protein | - |
Trichomonas vaginalis |
| H2 protein | - |
Trichomonas vaginalis |
| L protein | - |
Trichomonas vaginalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay condition for determination of the interaction of recombinant H an L protein | Trichomonas vaginalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.602 | - |
Lipoamide | kinetic parameter for L protein | Trichomonas vaginalis | |
| 0.84 | - |
rTvH1 protein | kinetic parameter for L protein | Trichomonas vaginalis | |
| 2.08 | - |
rTvH2 protein | kinetic parameter for L protein | Trichomonas vaginalis | |
| 6.08 | - |
Lipoamide | kinetic parameter for L protein | Trichomonas vaginalis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay condition for determination of the interaction of recombinant H an L protein | Trichomonas vaginalis |
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