Protein Variants | Comment | Organism |
---|---|---|
R229W | the mutation leads to deficiency of pyridoxal 5'-phosphate in the cell and causes neonatal epileptic encephalopathy | Homo sapiens |
R95C | the mutation leads to deficiency of pyridoxal 5'-phosphate in the cell and causes neonatal epileptic encephalopathy. The mutant exhibits a 15fold reduction in affinity for the FMN cofactor, a 71fold decrease in affinity for the substrate pyridoxine 5'-phosphate, a 4.9fold decrease in specific activity, and a 343fold reduction in catalytic activity, compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00618 | - |
pyridoxine 5'-phosphate | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens | |
0.436 | - |
pyridoxine 5'-phosphate | mutant enzyme R95C, at pH 8.0 and 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine 5'-phosphate + H2O + O2 | Homo sapiens | - |
pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
pyridoxine 5'-phosphate + O2 | Homo sapiens | - |
pyridoxal 5'-phosphate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NVS9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyridoxamine 5'-phosphate + H2O + O2 | - |
Homo sapiens | pyridoxal 5'-phosphate + NH3 + H2O2 | - |
? | |
pyridoxine 5'-phosphate + O2 | - |
Homo sapiens | pyridoxal 5'-phosphate + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PNPO | - |
Homo sapiens |
pyridoxine 5'-phosphate oxidase` | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
pyridoxine 5'-phosphate | mutant enzyme R95C, at pH 8.0 and 37°C | Homo sapiens | |
0.18 | - |
pyridoxine 5'-phosphate | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.085 | - |
pyridoxine 5'-phosphate | mutant enzyme R95C, at pH 8.0 and 37°C | Homo sapiens | |
29 | - |
pyridoxine 5'-phosphate | wild type enzyme, at pH 8.0 and 37°C | Homo sapiens |