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Literature summary for 1.4.3.5 extracted from

  • Tsuge, H.; Ozeki, K.; Ohashi, K.
    Molecular and enzymatic properties of pyridoxamine (pyridoxine) 5'-phosphate oxidase from baker's yeast (1980), Agric. Biol. Chem., 44, 2329-2335.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000016
-
FMN
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
1 * 27000 + 1 * 25000, SDS-PAGE Saccharomyces cerevisiae
27000
-
1 * 27000 + 1 * 25000, SDS-PAGE Saccharomyces cerevisiae
55000
-
analytical polyacrylamide disc gel electrophoresis Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0417
-
activity of apoenzyme after dialysis of holoenzyme against potassium acetate buffer pH 4.0 and potassium phosphate buffer pH 7.0 for 24 h each Saccharomyces cerevisiae
0.435
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + H2O + O2
-
Saccharomyces cerevisiae pyridoxal 5'-phosphate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
dimer 1 * 27000 + 1 * 25000, SDS-PAGE Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
FMN 1 mol of FMN per mol of enzyme Saccharomyces cerevisiae