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Literature summary for 1.4.3.5 extracted from

  • Horiike, K.; Merrill, A.H.; McCormick, D.B.
    Activation and inactivation of rabbit liver pyridoxamine (pyridoxine) 5-phosphate oxidase activity by urea and other solutes (1979), Arch. Biochem. Biophys., 195, 325-335.
    View publication on PubMed
Search for more literature for 1.4.3.5

Activating Compound

Activating Compound Comment Organism Structure
guanidine hydrochloride 500 mM, activation at low concentrations and brief periods of exposure, inactivation at higher concentrations and longer periods of exposure Oryctolagus cuniculus
guanidine hydrochloride urea both activates and denatures the enzyme Oryctolagus cuniculus
Urea 2.0-2.5 M, rapid and reversible activation at low concentrations and brief periods of exposure, inactivation at higher concentrations and longer periods of exposure Oryctolagus cuniculus
Urea both activates and denatures the enzyme, rapid and reversible activation at 2.0-2.5 M urea Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride urea both activates and denatures the enzyme Oryctolagus cuniculus
pyridoxal 5'-phosphate potent product inhibition Oryctolagus cuniculus
Urea both activates and denatures the enzyme, reversible inactivation above 2.5 M, half-time of inactivation is 46 min at 2 M and 37°C Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Oryctolagus cuniculus
0.0112
-
 pyridoxamine 5'-phosphate
-
 Oryctolagus cuniculus
0.0112
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus
0.0187
-
 pyridoxamine 5'-phosphate in the presence of 1 M urea Oryctolagus cuniculus
0.0187
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C, in presence of 1 M urea Oryctolagus cuniculus
0.0238
-
 pyridoxine 5'-phosphate
-
 Oryctolagus cuniculus
0.0238
-
 pyridoxine 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus
0.0417
-
 pyridoxamine 5'-phosphate in the presence of 2 M urea Oryctolagus cuniculus
0.0417
-
 pyridoxamine 5'-phosphate pH 8.0, 37°C, in presence of 2 M urea Oryctolagus cuniculus
0.0667
-
 pyridoxine 5'-phosphate in the presence of 2 M urea Oryctolagus cuniculus
0.0667
-
 pyridoxine 5'-phosphate pH 8.0, 37°C, in presence of 2 M urea Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information neutral salts, such as LiCl, KCl, NaCl, KBr, KI, and KSCN, both activate and denature the enzyme Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000 28000 gel filtration Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from liver Oryctolagus cuniculus

Renatured (Commentary)

Renatured (Comment) Organism
urea both activates and denatures the enzyme, reversible inactivation above 2.5 M, half-time of inactivation is 46 min at 2 M and 37°C Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyridoxamine 5'-phosphate + FMN + H2O + 2 H+
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + FMNH2 + NH3
-
 ?
pyridoxamine 5'-phosphate + H2O + O2
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
pyridoxine 5'-phosphate + FMN
-
 Oryctolagus cuniculus pyridoxal 5'-phosphate + FMNH2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000-28000, denaturing gel filtration Oryctolagus cuniculus

Synonyms

Synonyms Comment Organism
pyridoxamine (pyridoxine) 5'-phosphate oxidase
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
FMN bound, required Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics of urea, guanidinium hydrochloride, and neutral salts such as LiCl, KCl, NaCl, KBr, KI, and KSCN Oryctolagus cuniculus
0.019
-
 pyridoxal 5'-phosphate pH 8.0, 37°C Oryctolagus cuniculus
0.025
-
 pyridoxal 5'-phosphate pH 8.0, 37°C, in presence of 1 M urea Oryctolagus cuniculus
0.044
-
 pyridoxal 5'-phosphate pH 8.0, 37°C, in presence of 2 M urea Oryctolagus cuniculus