Literature summary for 1.4.3.5 extracted from

Churchich, J.E.
Brain pyridoxine-5-phosphate oxidase. A dimeric enzyme containing one FMN site (1984), Eur. J. Biochem., 138, 327-332.

Search for more literature for 1.4.3.5

Inhibitors

Inhibitors	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Sus scrofa
pyridoxaloxime 5'-phosphate	competitive inhibition	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01	-	5'-phospho-pyridoxyl-aniline	-	Sus scrofa
0.01	-	5'-phospho-pyridoxyl-3-aminobenzoate	-	Sus scrofa
0.01	-	5'-phospho-pyridoxyl-4-aminobenzoate	-	Sus scrofa
0.013	-	pyridoxine 5'-phosphate	-	Sus scrofa
0.03	-	pyridoxine 5'-phosphate	-	Sus scrofa
0.03	-	5'-phospho-pyridoxyl-4-aminobutyrate	-	Sus scrofa
0.035	-	5'-phospho-pyridoxyl-3-L-alanine	-	Sus scrofa
0.1	-	pyridoxamine-5'-phosphate	-	Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	2 * 30000, drastic denaturation conditions: 0.1% SDS and 2-mercaptoethanol, SDS-PAGE	Sus scrofa
60000	-	polyacrylamide gradient gel electrophoresis	Sus scrofa

Organism

Organism	UniProt	Comment	Textmining
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.09	-	-	Sus scrofa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-phospho-pyridoxyl-3-alanine + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + alanine + H2O2	-	?
5'-phospho-pyridoxyl-3-aminobenzoate + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + 3-aminobenzoate + H2O2	-	?
5'-phospho-pyridoxyl-3-L-alanine + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + L-alanine + H2O2	-	r
5'-phospho-pyridoxyl-4-aminobenzoate + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + 4-aminobenzoate + H2O2	-	?
5'-phospho-pyridoxyl-4-aminobutyrate + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + 4-aminobutyrate + H2O2	-	?
5'-phospho-pyridoxyl-4-aminonitrobenzene + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + 4-aminonitrobenzene + H2O2	-	?
5'-phospho-pyridoxyl-4-aminophenol + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + 4-aminophenol + H2O2	-	?
5'-phospho-pyridoxyl-5-aminovalerate + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + 5-aminovalerate + H2O2	-	?
5'-phospho-pyridoxyl-aniline + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + aniline + H2O2	-	?
pyridoxamine 5'-phosphate + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + NH3 + H2O2	-	?
pyridoxine 5'-phosphate + H2O + O2	-	Sus scrofa	pyridoxal 5'-phosphate + H2O2	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 30000, drastic denaturation conditions: 0.1% SDS and 2-mercaptoethanol, SDS-PAGE	Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.004	-	5'-phospho-pyridoxyl-p-aminophenol	-	Sus scrofa
0.009	-	pyridoxamine 5'-phosphate	-	Sus scrofa
0.01	-	5'-phospho-pyridoxyl-aniline	-	Sus scrofa
0.035	-	5'-phospho-pyridoxyl-5-aminovalerate	-	Sus scrofa
0.04	-	5'-phospho-pyridoxyl-3-alanine	-	Sus scrofa
0.04	-	5'-phospho-pyridoxyl-4-aminobutyrate	-	Sus scrofa
0.09	-	pyridoxine 5'-phosphate	-	Sus scrofa
0.1	-	5'-phospho-pyridoxyl-3-aminobenzoate	-	Sus scrofa
0.1	-	5'-phospho-pyridoxyl-p-aminonitrobenzene	-	Sus scrofa

Cofactor

Cofactor	Comment	Organism	Structure
FMN	flavoprotein	Sus scrofa
FMN	binds 1 mol of FMN per mol of dimer	Sus scrofa

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Release 2023.1 (January 2023)