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Literature summary for 1.4.3.4 extracted from

  • Milczek, E.M.; Binda, C.; Rovida, S.; Mattevi, A.; Edmondson, D.E.
    The gating residues Ile199 and Tyr326 in human monoamine oxidase B function in substrate and inhibitor recognition (2011), FEBS J., 278, 4860-4869.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
using 12% (w/v) polyethylene glycol 4000, 100 mM N-(2-acetamido)-2-iminodiacetic acid buffer pH 6.5, and 70 mM lithium sulfate Homo sapiens

Protein Variants

Protein Variants Comment Organism
I199A the mutant exhibits catalytic properties with 4fold increased amine Km but unaltered kcat values Homo sapiens
I199A/Y326A the mutant exhibits catalytic properties with 75fold increased amine Km but unaltered kcat values. The mutant shows inhibitor binding properties more similar to those of isoform MAO A than to isoform MAO B. Benzylamine is a poor substrate for the double mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,4-diphenyl-1,3-butadiene
-
 Homo sapiens
1,4-diphenyl-2-butene
-
 Homo sapiens
8-(3-chloro)styrylcaffeine
-
 Homo sapiens
farnesol
-
 Homo sapiens
Harmane
-
 Homo sapiens
isatin non-specific reversible inhibitor Homo sapiens
methylene blue
-
 Homo sapiens
additional information isoform MAO B is not inhibited by pirlindole Homo sapiens
safinamide
-
 Homo sapiens
tranylcypromine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0089
-
 phenylbutylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.0094
-
 Phenylethylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.019
-
 phenylbutylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.027
-
 kynuramine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.04
-
 Phenylethylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.055
-
 phenylbutylamine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.094
-
 kynuramine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.15
-
 benzylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.289
-
 kynuramine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.703
-
 Phenylethylamine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
2.133
-
 benzylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-[4-(trifluoromethyl)phenyl]methanamine + H2O + O2
-
 Homo sapiens 4-(trifluoromethyl)benzaldehyde + NH3 + H2O2
-
 ?
benzylamine + H2O + O2
-
 Homo sapiens benzaldehyde + NH3 + H2O2
-
 ?
kynuramine + H2O + O2
-
 Homo sapiens 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?
phenylbutylamine + H2O + O2
-
 Homo sapiens 2-phenylbutanal + NH3 + H2O2 + NH3 + H2O2
-
 ?
phenylethylamine + H2O + O2
-
 Homo sapiens 2-phenylethanal + NH3 + H2O2 + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
MAO B isoform Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.07
-
 kynuramine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
1.47
-
 kynuramine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
1.6
-
 kynuramine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
1.83
-
 Phenylethylamine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
1.83
-
 phenylbutylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
2.05
-
 Phenylethylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
2.25
-
 phenylbutylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
2.34
-
 phenylbutylamine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
2.87
-
 Phenylethylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
3.8
-
 benzylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
5
-
 benzylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000021
-
 safinamide mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.000143
-
 methylene blue mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.000214
-
 methylene blue mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.00027
-
 8-(3-chloro)styrylcaffeine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.0005
-
 safinamide wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.001
-
 1,4-diphenyl-1,3-butadiene mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.00101
-
 methylene blue wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.0017
-
 8-(3-chloro)styrylcaffeine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.0023
-
 farnesol wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.0027
-
 farnesol mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.003
-
 isatin wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.004
-
 safinamide mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.007
-
 1,4-diphenyl-1,3-butadiene wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.0081
-
 1,4-diphenyl-2-butene mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.011
-
 tranylcypromine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.012
-
 isatin mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.016
-
 tranylcypromine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.017
-
 tranylcypromine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.018
-
 farnesol mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.0345
-
 1,4-diphenyl-2-butene wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.14
-
 Harmane wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.165
-
 Harmane mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.36
-
 isatin mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
0.55
-
 Harmane mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.78
-
 benzylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
2.7
-
 Phenylethylamine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
3.7
-
 kynuramine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
15.6
-
 kynuramine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
33.3
-
 benzylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
43.3
-
 phenylbutylamine mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
51.7
-
 Phenylethylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
59.2
-
 kynuramine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
96.7
-
 phenylbutylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
253.3
-
 phenylbutylamine mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens
300
-
 Phenylethylamine wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) Homo sapiens