Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-bipyridyl | 50% inhibition at 1 mM | Narcissus tazetta | |
2-Bromoethylamine | complete inhibition at 0.01 mM | Narcissus tazetta | |
additional information | hardly inhibited by 1 mM o-phenanthroline (4%), pargyline (2%) and clorgyline (1%) | Narcissus tazetta | |
Semicarbazide | complete inhibition at 0.01 mM | Narcissus tazetta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
n-Propylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
0.11 | - |
methylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
0.11 | - |
n-butylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
0.13 | - |
ethylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
0.41 | - |
benzylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
0.5 | - |
n-Pentylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
1.4 | - |
2-Phenylethylamine | at pH 7.0 and 37°C | Narcissus tazetta | |
2 | - |
ethanolamine | at pH 7.0 and 37°C | Narcissus tazetta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme contains 2.44 mol of copper atoms per mole of enzyme | Narcissus tazetta | |
Mn2+ | 133% activity at 0.1 mM Mn2+ | Narcissus tazetta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
1 * 75000 + 1 * 65000, calculated from amino acid sequence | Narcissus tazetta |
75000 | - |
1 * 75000 + 1 * 65000, calculated from amino acid sequence | Narcissus tazetta |
135000 | - |
gel filtration | Narcissus tazetta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Narcissus tazetta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sephadex G-25 gel filtration, DEAE Sephacel gel filtration, and hydroxyapatite column chromatography | Narcissus tazetta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
shoot | - |
Narcissus tazetta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0032 | - |
crude extract, at pH 7.0 and 37°C | Narcissus tazetta |
1.365 | - |
1544fold purified enzyme, at pH 7.0 and 37°C | Narcissus tazetta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | 9% activity compared to n-propylamine | Narcissus tazetta | 2-phenylethanal + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | 74% activity compared to n-propylamine | Narcissus tazetta | benzaldehyde + NH3 + H2O2 | - |
? | |
ethanolamine + H2O + O2 | 50% activity compared to n-propylamine | Narcissus tazetta | glycolaldehyde + NH3 + H2O2 | - |
? | |
ethylamine + H2O + O2 | 84% activity compared to n-propylamine | Narcissus tazetta | ethanal + NH3 + H2O2 | - |
? | |
hexylamine + H2O + O2 | 7% activity compared to n-propylamine | Narcissus tazetta | hexanal + NH3 + H2O2 | - |
? | |
methylamine + H2O + O2 | 34% activity compared to n-propylamine | Narcissus tazetta | formaldehyde + NH3 + H2O2 | - |
? | |
additional information | no activity with tryptamine, serotonin, dopamine, tyramine, histamine, putrescine, cadaverine, diaminodecane, spermidine, and spermine | Narcissus tazetta | ? | - |
? | |
n-butylamine + H2O + O2 | 98% activity compared to n-propylamine | Narcissus tazetta | butyraldehyde + NH3 + H2O2 | - |
? | |
n-pentylamine + H2O + O2 | 56% activity compared to n-propylamine | Narcissus tazetta | pentaldehyde + NH3 + H2O2 | - |
? | |
n-propylamine + H2O + O2 | 100% activity | Narcissus tazetta | propionaldehyde + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 75000 + 1 * 65000, calculated from amino acid sequence | Narcissus tazetta |
Synonyms | Comment | Organism |
---|---|---|
MAO | - |
Narcissus tazetta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | the purified enzyme retains its activity at temperatures up to 70°C but loses activity quickly after pre-incubation at temperatures of 75°C (less than 50%) and higher | Narcissus tazetta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Narcissus tazetta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Narcissus tazetta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Narcissus tazetta |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
1 | - |
at pH 7.0 and 37°C | Narcissus tazetta | 2,2'-bipyridyl |