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Literature summary for 1.4.3.4 extracted from

  • Zhang, Y.; Livingstone, J.; Hirasawa, E.
    Purification and characterisation of monoamine oxidase from Avena sativa (2012), Acta Physiol. Plant., 34, 1411-1419.
No PubMed abstract available
Search for more literature for 1.4.3.4

Inhibitors

Inhibitors Comment Organism Structure
benzaldehyde strong competitive inhibitor Avena sativa
additional information enzyme activity is not inhibited by pargyline, clorgyline, semicarbazide, or sodium diethyldithiocarbamate Avena sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 benzylamine at pH 7.0 and 37°C Avena sativa
0.71
-
 2-Phenylethylamine at pH 7.0 and 37°C Avena sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa
55000
-
 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa
58000
-
 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa
78000
-
 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa
220000
-
 gel filtration Avena sativa

Organism

Organism UniProt Comment Textmining
Avena sativa
-
 cultivar Miechikara
-

Purification (Commentary)

Purification (Comment) Organism
Sephadex G-25 gel filtration, DEAE Sephacel gel filtration, hydroxyapatitecolumn chromatography, Mono Q column chromatography, and TSK-GEL filtration Avena sativa

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Avena sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00015
-
 crude extract, at pH 7.0 and 37°C Avena sativa
0.02
-
 125fold purified enzyme, at pH 7.0 and 37°C Avena sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethylamine + H2O + O2 the enzyme shows high substrate specificity for phenethylamine (100% activity) Avena sativa 2-phenylacetaldehyde + NH3 + H2O2
-
 ?
benzylamine + H2O + O2 60% activity compared to 2-phenylethylamine Avena sativa benzaldehyde + NH3 + H2O2
-
 ?
additional information no activity with tyramine, tryptamine, serotonin, dopamine, ethanolamine, histamine, ethylamine, n-propylamine, n-butylamine, n-pentylamine, putrescine, and spermidine Avena sativa ?
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 1 * 78000 + 1 * 58000 + 1 * 55000 + 1 * 32000, SDS-PAGE Avena sativa

Synonyms

Synonyms Comment Organism
MAO
-
 Avena sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Avena sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 80 the purified enzyme retains its activity at temperatures up to 50°C, but loses activity quickly after 10 min incubation at temperaturesabove 50°C Avena sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Avena sativa

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme contains 2 mol of FAD per 220000 g of enzyme Avena sativa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 benzaldehyde at pH 7.0 and 37°C Avena sativa

pI Value

Organism Comment pI Value Maximum pI Value
Avena sativa isoelectric focusing
-
 6.3

Expression

Organism Comment Expression
Avena sativa enzyme activity increases up to day 3 after germination, thereafter decreasing again up