Cloned (Comment) | Organism |
---|---|
functional expression of zMAO in Pichia pastoris mitochondria with the gene encoding zMAO under control of the methanol oxidase promotor | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.108 | - |
O2 | pH 7.4, 25°C, with benzylamine | Danio rerio | |
0.14 | - |
O2 | pH 7.4, 25°C, with phenylethylamine | Danio rerio | |
82 | - |
benzylamine | pH 7.4, 25°C | Danio rerio | |
86 | - |
2-Phenylethylamine | pH 7.4, 25°C | Danio rerio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Danio rerio | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58525 | - |
x * 58525, mass spectrometry, x * 60000, recombinant enzyme, SDS-PAGE | Danio rerio |
60000 | - |
x * 58525, mass spectrometry, x * 60000, recombinant enzyme, SDS-PAGE | Danio rerio |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | Danio rerio | - |
benzaldehyde + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant zMAO from Pichia pastoris mitochondria to homogeneity from membranes by treatment with phospholipase A and Triton X-100, ultracentrifugation, and hydroxyapatite chromatography | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | - |
Danio rerio | 2-phenylethanal + NH3 + H2O2 | - |
? | |
benzylamine + H2O + O2 | - |
Danio rerio | benzaldehyde + NH3 + H2O2 | - |
? | |
kynuramine + O2 | - |
Danio rerio | 4-hydroxyquinoline + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58525, mass spectrometry, x * 60000, recombinant enzyme, SDS-PAGE | Danio rerio |
More | zMAO peptide mapping using tryptic digestion, overview | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
zMAO | - |
Danio rerio |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
benzylamine | pH 7.4, 25°C | Danio rerio | |
0.093 | - |
O2 | pH 7.4, 25°C, with benzylamine | Danio rerio | |
3.4 | - |
2-Phenylethylamine | pH 7.4, 25°C | Danio rerio | |
5.4 | - |
O2 | pH 7.4, 25°C, with phenylethylamine | Danio rerio |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Danio rerio |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one mol of covalent flavin cofactor per mol of enzyme | Danio rerio |
General Information | Comment | Organism |
---|---|---|
additional information | zMAO exhibits no immuno-chemical cross-reactivity with polyclonal anti-sera raised against human MAO-A | Danio rerio |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
benzylamine | pH 7.4, 25°C | Danio rerio | |
0.4 | - |
2-Phenylethylamine | pH 7.4, 25°C | Danio rerio |