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Literature summary for 1.4.3.4 extracted from
- Expression of zebrafish (Danio rerio) monoamine oxidase (MAO) in Pichia pastoris: purification and comparison with human MAO A and MAO B (2010), Protein Expr. Purif., 70, 290-297.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of zMAO in Pichia pastoris mitochondria with the gene encoding zMAO under control of the methanol oxidase promotor | Danio rerio |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.108 | - |
O2 | pH 7.4, 25°C, with benzylamine | Danio rerio |  |
| 0.14 | - |
O2 | pH 7.4, 25°C, with phenylethylamine | Danio rerio | |
| 82 | - |
benzylamine | pH 7.4, 25°C | Danio rerio | |
| 86 | - |
2-Phenylethylamine | pH 7.4, 25°C | Danio rerio | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Danio rerio | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58525 | - |
x * 58525, mass spectrometry, x * 60000, recombinant enzyme, SDS-PAGE | Danio rerio |
| 60000 | - |
x * 58525, mass spectrometry, x * 60000, recombinant enzyme, SDS-PAGE | Danio rerio |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzylamine + H2O + O2 | Danio rerio | - |
benzaldehyde + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant zMAO from Pichia pastoris mitochondria to homogeneity from membranes by treatment with phospholipase A and Triton X-100, ultracentrifugation, and hydroxyapatite chromatography | Danio rerio |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-phenylethylamine + H2O + O2 | - |
Danio rerio | 2-phenylethanal + NH3 + H2O2 | - |
? | |
| benzylamine + H2O + O2 | - |
Danio rerio | benzaldehyde + NH3 + H2O2 | - |
? | |
| kynuramine + O2 | - |
Danio rerio | 4-hydroxyquinoline + NH3 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58525, mass spectrometry, x * 60000, recombinant enzyme, SDS-PAGE | Danio rerio |
| More | zMAO peptide mapping using tryptic digestion, overview | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| zMAO | - |
Danio rerio |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Danio rerio |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.078 | - |
benzylamine | pH 7.4, 25°C | Danio rerio | |
| 0.093 | - |
O2 | pH 7.4, 25°C, with benzylamine | Danio rerio | |
| 3.4 | - |
2-Phenylethylamine | pH 7.4, 25°C | Danio rerio | |
| 5.4 | - |
O2 | pH 7.4, 25°C, with phenylethylamine | Danio rerio | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Danio rerio |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | one mol of covalent flavin cofactor per mol of enzyme | Danio rerio | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | zMAO exhibits no immuno-chemical cross-reactivity with polyclonal anti-sera raised against human MAO-A | Danio rerio |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0095 | - |
benzylamine | pH 7.4, 25°C | Danio rerio | |
| 0.4 | - |
2-Phenylethylamine | pH 7.4, 25°C | Danio rerio | |
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