Literature summary for 1.4.3.3 extracted from

Abad, S.; Nahalka, J.; Winkler, M.; Bergler, G.; Speight, R.; Glieder, A.; Nidetzky, B.
High-level expression of Rhodotorula gracilis D: -amino acid oxidase in Pichia pastoris (2011), Biotechnol. Lett., 33, 557-563.

Search for more literature for 1.4.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris strain CBS 7435, the enzyme production is enhanced to 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h, subcloning in Escherichia coli. Expression optimzation and quantitative real-time PCR analysis expression analysis,overview	Rhodotorula toruloides

Protein Variants

Protein Variants	Comment	Organism
additional information	establishing the transformed Pichia pastoris, expressing RgDAO, as whole-cell catalyst	Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-methionine + H2O + O2	Rhodotorula toruloides	-	4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhodotorula toruloides	P80324	DAO1; i.e. Rhodosporidium toruloides	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant DAo from Pichia pastoris strain CBS 7435, the yeast cells are permeabilized by freeze-drying and incubation in 10% v/v 2-propanol resulting in a highly active, 1.6 kUnits/g dry matter, and stable oxidase preparation	Rhodotorula toruloides

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-methionine + H2O + O2	-	Rhodotorula toruloides	4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
DAO	-	Rhodotorula toruloides

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Release 2023.1 (January 2023)