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Literature summary for 1.4.3.3 extracted from
- Characterization of a yeast D-amino acid oxidase microbiosensor for D-serine detection in the central nervous system (2008), Anal. Chem., 80, 1589-1597.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Rhodotorula toruloides |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.62 | - |
D-Ser | apparent value, immobilized enzyme | Rhodotorula toruloides |  |
| 13.75 | - |
D-Ser | apparent value, free enzyme | Rhodotorula toruloides | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula toruloides | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodotorula toruloides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, immobilized on microbiosensor, more than 160 days, 2% loss of activity | Sus scrofa |
| -20°C, immobilized on microbiosensor, more than 160 days, 2% loss of activity | Rhodotorula toruloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala + H2O + O2 | 104% activity compared to D-Ser | Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
| D-Ala + H2O + O2 | 165% activity compared to D-Ser | Sus scrofa | pyruvate + NH3 + H2O2 | - |
? | |
| D-Asp + H2O + O2 | 38.6% activity compared to D-Ser | Sus scrofa | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-Asp + H2O + O2 | 5.5% activity compared to D-Ser | Rhodotorula toruloides | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-Ser + H2O + O2 | 100% activity | Sus scrofa | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
| D-Ser + H2O + O2 | 100% activity | Rhodotorula toruloides | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-amino acid oxidase | - |
Sus scrofa |
| D-amino acid oxidase | - |
Rhodotorula toruloides |
| DAAO | - |
Sus scrofa |
| DAAO | - |
Rhodotorula toruloides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 40 | the response to D-serine increases with temperature, from 15 to 40°C, the sensitivity at 37°C is 75% higher than that at 25°C | Rhodotorula toruloides |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | immobilized enzyme | Rhodotorula toruloides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | 8.5 | the response to 0.01 mM D-serine increases from pH 5.4 to 8.0, reaches a maximum at pH 8.0-8.5, and decreases at higher values, the response at physiological pH (7.4) is 80.7% of the maximum response observed at pH 8.5 | Rhodotorula toruloides |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Sus scrofa | |
| FAD | - |
Rhodotorula toruloides | |
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