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Literature summary for 1.4.3.3 extracted from

  • Bakke, M.; Setoyama, C.; Miura, R.; Kajiyama, N.
    Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution (2006), Biotechnol. Bioeng., 93, 1023-1027.
    View publication on PubMed
Search for more literature for 1.4.3.3

Protein Variants

Protein Variants Comment Organism
F42C mutant retains more than 70% of activity after 1 h, while the wild-type enzyme retains only 10% activity. Optimal temperature of mutant enzyme is about 10°C higher than that of wild-type enzyme. Activity at the optimal temperature is about 20% higher than that of wild-type enzyme. KM-values for D-Ala, D-Met, D-Phe and D-Ser are 40-50% of the wild-type value Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 D-Met pH 8.3, 37°C, mutant enzyme F42C Sus scrofa
0.9
-
 D-Met pH 8.3, 37°C, wild-type enzyme Sus scrofa
1.2
-
 D-Ala pH 8.3, 37°C, mutant enzyme F42C Sus scrofa
1.9
-
 D-Phe pH 8.3, 37°C, mutant enzyme F42C Sus scrofa
2.5
-
 D-Ala pH 8.3, 37°C, wild-type enzyme Sus scrofa
3.6
-
 D-Phe pH 8.3, 37°C, wild-type enzyme Sus scrofa
4.7
-
 D-Ser pH 8.3, 37°C, mutant enzyme F42C Sus scrofa
12.7
-
 D-Ser pH 8.3, 37°C, wild-type enzyme Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala + H2O + O2
-
 Sus scrofa pyruvate + NH3 + H2O2
-
 ?
D-Met + H2O + O2
-
 Sus scrofa 4-methylthio-2-oxobutanoate + NH3 + H2O2
-
 ?
D-Phe + H2O + O2
-
 Sus scrofa phenylpyruvate + NH3 + H2O2
-
 ?
D-Ser + H2O + O2
-
 Sus scrofa 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50 wild-type enzyme Sus scrofa
55
-
 mutant enzyme F42C Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 50% of maximal activity, 60°C: about 40% of maximal activity, wild-type enzyme Sus scrofa
40 70 40°C and 70°C: about 60% of maximal activity, mutant enzyme F42C Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 wild-type enzyme is unstable above Sus scrofa
55
-
 1 h, mutant enzyme F42C retains more than 70% of activity, wild-type enzyme retains only 10% activity Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10 wild-type enzyme and mutant enzyme F42C Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
7.5 11 pH 7.5 and pH 11: about 40% of maximal activity Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 25°C, 24 h, stable, wild-type enzyme and mutant enzyme F42C Sus scrofa