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Literature summary for 1.4.3.3 extracted from

  • Pollegioni, L.; Caldinelli, L.; Molla, G.; Sacchi, S.; Pilone, M.S.
    Catalytic properties of D-amino acid oxidase in cephalosporin C bioconversion: a comparison between proteins from different sources (2004), Biotechnol. Prog., 20, 467-473.
    View publication on PubMed
Search for more literature for 1.4.3.3

Inhibitors

Inhibitors Comment Organism Structure
7-(5-oxoadipoamido)cephalosporanic acid product inhibition Rhodotorula toruloides
7-(5-oxoadipoamido)cephalosporanic acid product inhibition Sus scrofa
7-(5-oxoadipoamido)cephalosporanic acid product inhibition Trigonopsis variabilis
glutaryl-7-aminocephalosporanic acid noncompetitive Rhodotorula toruloides
glutaryl-7-aminocephalosporanic acid
-
 Sus scrofa
glutaryl-7-aminocephalosporanic acid noncompetitive Trigonopsis variabilis
H2O2 product inhibition, competitive versus cephalosporin Rhodotorula toruloides
H2O2 product inhibition, noncompetitive versus cephalosporin Sus scrofa
H2O2 product inhibition, competitive versus cephalosporin Trigonopsis variabilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
 D-Ala pH 8.5, 25°C Rhodotorula toruloides
1.7
-
 D-Ala pH 8.5, 25°C Sus scrofa
2.3
-
 cephalosporin C pH 8.5, 25°C Sus scrofa
2.4
-
 cephalosporin C pH 8.5, 25°C Trigonopsis variabilis
4
-
 cephalosporin C pH 8.5, 25°C Rhodotorula toruloides
7
-
 D-Ala pH 8.5, 25°C Trigonopsis variabilis

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides
-
-
-
Sus scrofa
-
-
-
Trigonopsis variabilis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
 Sus scrofa 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
cephalosporin C + H2O + O2
-
 Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
cephalosporin C + H2O + O2
-
 Trigonopsis variabilis 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
D-Ala + H2O + O2
-
 Sus scrofa pyruvate + NH3 + H2O2
-
 ?
D-Ala + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
D-Ala + H2O + O2
-
 Trigonopsis variabilis pyruvate + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
DAAO
-
 Sus scrofa
DAAO
-
 Rhodotorula toruloides
DAAO
-
 Trigonopsis variabilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 30 min, about 30% loss of activity Sus scrofa
35
-
 30 min, about 30% loss of activity Rhodotorula toruloides
40
-
 30 min, stable Trigonopsis variabilis
40
-
 30 min, about 40% loss of activity Sus scrofa
40
-
 30 min, about 95% loss of activity Rhodotorula toruloides
45
-
 30 min, about 50% loss of activity Sus scrofa
45
-
 30 min, about 15% loss of activity Trigonopsis variabilis
50
-
 30 min, about 75% loss of activity Sus scrofa
55
-
 30 min, complete loss of activity Sus scrofa
55
-
 30 min, about 55% loss of activity Trigonopsis variabilis
60
-
 30 min, about 80% loss of activity Trigonopsis variabilis
65
-
 30 min, complete loss of activity Trigonopsis variabilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
39
-
 cephalosporin C pH 8.5, 25°C Sus scrofa
440
-
 D-Ala pH 8.5, 25°C Sus scrofa
2740
-
 D-Ala pH 8.5, 25°C Trigonopsis variabilis
4300
-
 cephalosporin C pH 8.5, 25°C Trigonopsis variabilis
4400
-
 cephalosporin C pH 8.5, 25°C Rhodotorula toruloides
4880
-
 D-Ala pH 8.5, 25°C Rhodotorula toruloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Trigonopsis variabilis
9
-
-
 Rhodotorula toruloides
9 10
-
 Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10 pH 6.5: about 55% of maximal activity, pH 10.0: about 50% of maximal activity Trigonopsis variabilis
6.5 10 pH 6.5: about 55% of maximal activity, pH 10.0: about 80% of maximal activity Rhodotorula toruloides
8.4 10 pH 8.4: about 60% of maximal activity, pH 9.0-10.0: maximum Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
9.5
-
 maximal stability Sus scrofa
9.5
-
 maximal stability Rhodotorula toruloides
9.5
-
 maximal stability Trigonopsis variabilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.16 1.4 glutaryl-7-aminocephalosporanic acid pH 8.5, 25°C Sus scrofa
3.9
-
 H2O2 pH 8.5, 25°C Sus scrofa
8.1 25.3 glutaryl-7-aminocephalosporanic acid pH 8.5, 25°C Trigonopsis variabilis
10 14.1 7-(5-oxoadipoamido)cephalosporanic acid pH 8.5, 25°C Sus scrofa
11.8 26.5 glutaryl-7-aminocephalosporanic acid pH 8.5, 25°C Rhodotorula toruloides
23.6
-
 7-(5-oxoadipoamido)cephalosporanic acid pH 8.5, 25°C Rhodotorula toruloides
27.2
-
 7-(5-oxoadipoamido)cephalosporanic acid pH 8.5, 25°C Trigonopsis variabilis
33.1
-
 H2O2 pH 8.5, 25°C Rhodotorula toruloides
36
-
 H2O2 pH 8.5, 25°C Trigonopsis variabilis