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Literature summary for 1.4.3.3 extracted from
- Characterization of D-amino acid oxidase from Trigonopsis variabilis (1993), Biochem. Mol. Biol. Int., 31, 709-717.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes | Trigonopsis variabilis |
| stable to guanidine | Trigonopsis variabilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| anthranilate | - |
Trigonopsis variabilis |  |
| benzoic acid | competitive inhibitor | Trigonopsis variabilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.95 | - |
O2 | cephalosporin C | Trigonopsis variabilis | |
| 1.02 | - |
O2 | D-alanine | Trigonopsis variabilis | |
| 23 | - |
cephalosporin C | - |
Trigonopsis variabilis | |
| 43 | - |
D-alanine | - |
Trigonopsis variabilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
alpha2, 2 * 40000, SDS-PAGE | Trigonopsis variabilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trigonopsis variabilis | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | non-glycosylated | Trigonopsis variabilis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trigonopsis variabilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 86.6 | - |
- |
Trigonopsis variabilis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | alpha2, 2 * 40000, SDS-PAGE | Trigonopsis variabilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Trigonopsis variabilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 100 | - |
cephalosporin C | - |
Trigonopsis variabilis | |
| 300 | - |
D-alanine | - |
Trigonopsis variabilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Trigonopsis variabilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | - |
Trigonopsis variabilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1 FAD per monomer of MW 38000-39000 | Trigonopsis variabilis | |
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Release 2023.1 (January 2023)
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