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Literature summary for 1.4.3.23 extracted from

  • Nishizawa, T.; Aldrich, C.C.; Sherman, D.H.
    Molecular analysis of the rebeccamycin L-amino acid oxidase from Lechevalieria aerocolonigenes ATCC 39243 (2005), J. Bacteriol., 187, 2084-2092.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lentzea aerocolonigenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
7-chloro-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes
1.43
-
1-methyl-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes
1.53
-
L-Trp pH 7.8, 30°C Lentzea aerocolonigenes
1.84
-
5-fluoro-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53412
-
2 * 53412, calculated from sequence Lentzea aerocolonigenes
110000
-
gel filtration Lentzea aerocolonigenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7-chloro-L-tryptophan + O2 Lentzea aerocolonigenes rebeccamycin biosynthetic pathway 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Lentzea aerocolonigenes Q8KHS0 ATCC 39243
-

Purification (Commentary)

Purification (Comment) Organism
-
Lentzea aerocolonigenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-methyl-L-tryptophan + O2
-
Lentzea aerocolonigenes ?
-
?
5-fluoro-L-tryptophan + O2
-
Lentzea aerocolonigenes ?
-
?
5-methyl-DL-tryptophan + O2
-
Lentzea aerocolonigenes ?
-
?
7-chloro-L-tryptophan + O2 rebeccamycin biosynthetic pathway Lentzea aerocolonigenes 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2
-
?
7-chloro-L-tryptophan + O2 RebO is specific for the L-Trp enantiomer. 7-chloro-L-Trp is the preferred substrate Lentzea aerocolonigenes 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2
-
?
L-Trp + O2
-
Lentzea aerocolonigenes ?
-
?
additional information L-tryptophanamide and N-acetyl-tryptophan are not accepted as substrates by RebO Lentzea aerocolonigenes ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 53412, calculated from sequence Lentzea aerocolonigenes

Synonyms

Synonyms Comment Organism
RebO
-
Lentzea aerocolonigenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0255
-
1-methyl-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes
0.202
-
L-Trp pH 7.8, 30°C Lentzea aerocolonigenes
0.239
-
5-fluoro-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes
0.664
-
7-chloro-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes

Cofactor

Cofactor Comment Organism Structure
FAD contains noncovalently bound flavin of 0.58 mol per mol of protein. RebO protein that is overexpressed without supplementation of FAD in Escherichia coli BL21(DE3) contains less than 0.5 mol of noncovalently bound flavin per mol of enzyme Lentzea aerocolonigenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0178
-
1-methyl-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes
0.13
-
5-fluoro-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes
0.132
-
L-Trp pH 7.8, 30°C Lentzea aerocolonigenes
7.55
-
7-chloro-L-tryptophan pH 7.8, 30°C Lentzea aerocolonigenes