Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lentzea aerocolonigenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
7-chloro-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes | |
1.43 | - |
1-methyl-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes | |
1.53 | - |
L-Trp | pH 7.8, 30°C | Lentzea aerocolonigenes | |
1.84 | - |
5-fluoro-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53412 | - |
2 * 53412, calculated from sequence | Lentzea aerocolonigenes |
110000 | - |
gel filtration | Lentzea aerocolonigenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-chloro-L-tryptophan + O2 | Lentzea aerocolonigenes | rebeccamycin biosynthetic pathway | 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentzea aerocolonigenes | Q8KHS0 | ATCC 39243 | - |
Purification (Comment) | Organism |
---|---|
- |
Lentzea aerocolonigenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methyl-L-tryptophan + O2 | - |
Lentzea aerocolonigenes | ? | - |
? | |
5-fluoro-L-tryptophan + O2 | - |
Lentzea aerocolonigenes | ? | - |
? | |
5-methyl-DL-tryptophan + O2 | - |
Lentzea aerocolonigenes | ? | - |
? | |
7-chloro-L-tryptophan + O2 | rebeccamycin biosynthetic pathway | Lentzea aerocolonigenes | 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2 | - |
? | |
7-chloro-L-tryptophan + O2 | RebO is specific for the L-Trp enantiomer. 7-chloro-L-Trp is the preferred substrate | Lentzea aerocolonigenes | 2-imino-3-(7-chloroindol-3-yl)propanoate + H2O2 | - |
? | |
L-Trp + O2 | - |
Lentzea aerocolonigenes | ? | - |
? | |
additional information | L-tryptophanamide and N-acetyl-tryptophan are not accepted as substrates by RebO | Lentzea aerocolonigenes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53412, calculated from sequence | Lentzea aerocolonigenes |
Synonyms | Comment | Organism |
---|---|---|
RebO | - |
Lentzea aerocolonigenes |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0255 | - |
1-methyl-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes | |
0.202 | - |
L-Trp | pH 7.8, 30°C | Lentzea aerocolonigenes | |
0.239 | - |
5-fluoro-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes | |
0.664 | - |
7-chloro-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains noncovalently bound flavin of 0.58 mol per mol of protein. RebO protein that is overexpressed without supplementation of FAD in Escherichia coli BL21(DE3) contains less than 0.5 mol of noncovalently bound flavin per mol of enzyme | Lentzea aerocolonigenes |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0178 | - |
1-methyl-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes | |
0.13 | - |
5-fluoro-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes | |
0.132 | - |
L-Trp | pH 7.8, 30°C | Lentzea aerocolonigenes | |
7.55 | - |
7-chloro-L-tryptophan | pH 7.8, 30°C | Lentzea aerocolonigenes |