General Stability | Organism |
---|---|
purified enzyme does not withstand lyophilization | Euphorbia characias |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Arcaine sulfate | - |
Euphorbia characias | |
cyanide | - |
Euphorbia characias | |
diethyldithiocarbamate | - |
Euphorbia characias | |
Diethylthiocarbamate | - |
Euphorbia characias | |
histamine | - |
Euphorbia characias | |
isoniazid | - |
Euphorbia characias | |
NaCN | - |
Euphorbia characias | |
NaN3 | - |
Euphorbia characias | |
phenylhydrazine | - |
Euphorbia characias | |
Semicarbazide | - |
Euphorbia characias |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
putrescine | - |
Euphorbia characias | |
0.2 | - |
1,4-diaminobutane | - |
Euphorbia characias | |
0.47 | - |
cadaverine | - |
Euphorbia characias | |
0.47 | - |
1,5-Diaminopentane | - |
Euphorbia characias |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | enzyme contains 2 Cu2+ per dimer | Euphorbia characias | |
Manganese | no manganese found | Euphorbia characias |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
2 * 72000, SDS-PAGE | Euphorbia characias |
140000 | - |
gel filtration | Euphorbia characias |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euphorbia characias | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 12% neutral sugar | Euphorbia characias |
Purification (Comment) | Organism |
---|---|
- |
Euphorbia characias |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
- |
Euphorbia characias |
Storage Stability | Organism |
---|---|
-20°C, in water or potassium phosphate buffer, indefinitely stable | Euphorbia characias |
4°C, 50% loss of activity after 15 days | Euphorbia characias |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,10-diaminodecane + H2O + O2 | - |
Euphorbia characias | 10-aminodecanal + NH3 + H2O2 | - |
? | |
1,4-diaminobutane + H2O + O2 | - |
Euphorbia characias | 4-aminobutanal + NH3 + H2O2 | - |
? | |
1,5-diaminopentane + H2O + O2 | - |
Euphorbia characias | 5-aminopentanal + NH3 + H2O2 | - |
? | |
1,6-diaminohexane + H2O + O2 | - |
Euphorbia characias | 6-aminohexanal + NH3 + H2O2 | - |
? | |
1,7-diaminoheptane + H2O + O2 | - |
Euphorbia characias | 7-aminoheptanal + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | no activity with spermidine | Euphorbia characias | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | no activity with benzylamine | Euphorbia characias | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | no activity with p-dimethylaminomethylbenzylamine | Euphorbia characias | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | no activity with histamine | Euphorbia characias | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | only aliphatic diamines from C4 to C10 and cystamine are oxidized, enzymatic activity decreases sharply with increasing chain length of diamines | Euphorbia characias | RCHO + NH3 + H2O2 | - |
? | |
spermine + H2O + O2 | no reaction | Euphorbia characias | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 72000, SDS-PAGE | Euphorbia characias |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
phosphate buffer | Euphorbia characias |
7.6 | - |
in Tris buffer | Euphorbia characias |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | contains 2 carbonyl-like groups per dimer | Euphorbia characias |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00025 | - |
phenylhydrazine | - |
Euphorbia characias | |
0.004 | - |
Arcaine sulfate | - |
Euphorbia characias | |
0.05 | - |
Semicarbazide | - |
Euphorbia characias | |
0.12 | - |
NaCN | - |
Euphorbia characias | |
0.43 | - |
Diethylthiocarbamate | - |
Euphorbia characias | |
6 | - |
histamine | - |
Euphorbia characias | |
6 | - |
isoniazid | - |
Euphorbia characias | |
54 | - |
NaN3 | - |
Euphorbia characias |