Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae | Ogataea angusta |
Crystallization (Comment) | Organism |
---|---|
in complex with ethylamine and benzylamine, hanging drop vapor diffusion method, using 8-9% (w/v) polyethylene glycol 8000 in 0.22-0.25 M potassium phosphate (pH 6.0) | Ogataea angusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ogataea angusta | the catalytic mechanism can be divided into two half-reactions: a reductive half-reaction in which a primary amine substrate is oxidized to its corresponding aldehyde with the concomitant reduction of the organic cofactor 2,4,5-trihydroxyphenylalanine quinone and an oxidative half-reaction in which reduced 2,4,5-trihydroxyphenylalanine quinone is re-oxidized with the reduction of molecular oxygen to hydrogen peroxide | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | P12807 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | - |
Ogataea angusta | benzaldehyde + NH3 + H2O2 | - |
? | |
ethylamine + H2O + O2 | - |
Ogataea angusta | acetaldehyde + NH3 + H2O2 | - |
? | |
additional information | the catalytic mechanism can be divided into two half-reactions: a reductive half-reaction in which a primary amine substrate is oxidized to its corresponding aldehyde with the concomitant reduction of the organic cofactor 2,4,5-trihydroxyphenylalanine quinone and an oxidative half-reaction in which reduced 2,4,5-trihydroxyphenylalanine quinone is re-oxidized with the reduction of molecular oxygen to hydrogen peroxide | Ogataea angusta | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAO | - |
Ogataea angusta |
Copper amine oxidase | - |
Ogataea angusta |
hPAO-1 | - |
Ogataea angusta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
benzylamine | in 100 mM potassium phosphate, pH 7.2, temperature not specified in the publication | Ogataea angusta | |
20 | - |
ethylamine | in 100 mM potassium phosphate, pH 7.2, temperature not specified in the publication | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,4,5-trihydroxyphenylalanine quinone | - |
Ogataea angusta |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
benzylamine | in 100 mM potassium phosphate, pH 7.2, temperature not specified in the publication | Ogataea angusta | |
52 | - |
ethylamine | in 100 mM potassium phosphate, pH 7.2, temperature not specified in the publication | Ogataea angusta |