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Literature summary for 1.4.3.21 extracted from

  • Chang, C.M.; Klema, V.J.; Johnson, B.J.; Mure, M.; Klinman, J.P.; Wilmot, C.M.
    Kinetic and structural analysis of substrate specificity in two copper amine oxidases from Hansenula polymorpha (2010), Biochemistry, 49, 2540-2550.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae INVSC-1 cells Ogataea angusta

Crystallization (Commentary)

Crystallization (Comment) Organism
isoform HPAO-2, sitting drop vapor diffusion method, using 0.5-0.75 M potassium sodium tartrate tetrahydrate in 0.10 M phosphate, pH 6.0-7.5, at 20°C Ogataea angusta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
x * 72000, SDS-PAGE Ogataea angusta

Organism

Organism UniProt Comment Textmining
Ogataea angusta P12807
-
-

Purification (Commentary)

Purification (Comment) Organism
Q sepharose column chromatography Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylamine + H2O + O2
-
Ogataea angusta benzaldehyde + NH3 + H2O2
-
?
methylamine + H2O + O2
-
Ogataea angusta formaldehyde + NH3 + H2O2
-
?
additional information isoform HPAO-2 shows a clear preference for bulkier aromatic amines and isoform HPAO-1 shows a preference for short aliphatic amines Ogataea angusta ?
-
?

Subunits

Subunits Comment Organism
? x * 72000, SDS-PAGE Ogataea angusta

Synonyms

Synonyms Comment Organism
CAO
-
Ogataea angusta
Copper amine oxidase
-
Ogataea angusta
hPAO-1 isoform Ogataea angusta
HPAO-2 isoform Ogataea angusta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.066
-
benzylamine isoform HPAO-1, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
2.18
-
methylamine isoform HPAO-2, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
6.2
-
methylamine isoform HPAO-1, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
8.1
-
benzylamine isoform HPAO-2, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
2,4,5-trihydroxyphenylalanine quinone
-
Ogataea angusta

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.09
-
benzylamine isoform HPAO-1, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
1.2
-
methylamine isoform HPAO-2, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
30
-
methylamine isoform HPAO-1, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
81
-
O2 isoform HPAO-1, using benzylamine as cosubstrate, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
160
-
O2 isoform HPAO-2, using methylamine as cosubstrate, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
400
-
O2 isoform HPAO-1, using methylamine as cosubstrate, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
470
-
O2 isoform HPAO-2, using benzylamine as cosubstrate, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta
900
-
benzylamine isoform HPAO-2, at 25°C, in 100 mM potassium phosphate, pH 7.2 Ogataea angusta