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Literature summary for 1.4.3.2 extracted from
- Biochemical, biological and molecular characterization of an L-Amino acid oxidase (LAAO) purified from Bothrops pictus Peruvian snake venom (2017), Toxicon, 139, 74-86 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Bothrops pictus |  |
| glutathione | - |
Bothrops pictus | |
| iodoacetate | - |
Bothrops pictus | |
| additional information | not inhibitory: Ca2+, Mg2+ and Mn2+, PMSF, EDTA and glutamic acid | Bothrops pictus | |
| Zn2+ | - |
Bothrops pictus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 132000 | - |
gel filtration | Bothrops pictus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bothrops pictus | X2L4E2 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bothrops pictus |
| proteolytic modification | sequence contains a signal peptide of 11 amino acids | Bothrops pictus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by two chromatographic steps on Sephadex G-100 and CM Sephadex C-50 | Bothrops pictus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| venom | - |
Bothrops pictus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-leucine + H2O + O2 | - |
Bothrops pictus | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 65000, SDS-PAGE | Bothrops pictus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | - |
Bothrops pictus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
rapid loss of activity above | Bothrops pictus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
stable up to | Bothrops pictus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bothrops pictus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | - |
Bothrops pictus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Bothrops pictus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is evolutively close related to LAAOs from Bothrops jararacussu, Bothrops moojeni and Bothrops atrox, and together with the LAAO from Bothrops pauloensis, they form a well-defined cluster of the Bothrops genus | Bothrops pictus |
| additional information | the enzyme induces edema in mice, inhibits human platelet aggregation induced by ADP in a dose-dependent manner and shows antibacterial activity on Gram-positive and Gram-negative bacteria | Bothrops pictus |
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