Literature summary for 1.4.3.2 extracted from

Kasai, K.; Ito, Y.; Nitta, A.; Ariyoshi, K.; Nakamura, T.; Miura, T.
Metal coordination by L-amino acid oxidase derived from flounder Platichthys stellatus is structurally essential and regulates antibacterial activity (2020), Appl. Microbiol. Biotechnol., 104, 9645-9654 .

Search for more literature for 1.4.3.2

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Platichthys stellatus

Crystallization (Commentary)

Crystallization (Comment)	Organism
homology modeling of LAAO1 predicts metal coordination by residues Y241, H348, and D406	Platichthys stellatus

Protein Variants

Protein Variants	Comment	Organism
H209A	non-metal coordinating mutant, the levels of iron, zinc, and magnesium are similar to those of wild-type	Platichthys stellatus
H348A	contrary to wild-type, no magnesium ion is detected	Platichthys stellatus
H348A	mutant does not display antibacterial activity	Platichthys stellatus

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	the Michaelis constant of LAAO1 decreases and the catalytic constant (Kcat/Km) value increases following pre-treatment with EDTA. EDTA pre-chelated LAAO1 is more active than the non-EDTA treated LAAO1 sample against Staphylococcus aureus ATCC 25923, MRSA clinical isolate 87-7927, and Vibrio parahaemolyticus RIMD2210001	Platichthys stellatus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
13	-	L-lysine	pH 7, 37°C, EDTA-treated protein	Platichthys stellatus
67.7	-	L-lysine	pH 7, 37°C, untreated protein	Platichthys stellatus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	the mole ratios of one wild-type protein to iron, zinc, and magnesium are 0.12, 0.50, and 3.61, respectively	Platichthys stellatus
Magnesium	the mole ratios of one wild-type protein to iron, zinc, and magnesium are 0.12, 0.50, and 3.61, respectively	Platichthys stellatus
Zinc	the mole ratios of one wild-type protein to iron, zinc, and magnesium are 0.12, 0.50, and 3.61, respectively	Platichthys stellatus

Organism

Organism	UniProt	Comment	Textmining
Platichthys stellatus	D2KWB6	isoform LAAO-1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-lysine + H2O + O2	-	Platichthys stellatus	6-amino-2-oxohexanoate + NH3 + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
LAAO-1	-	Platichthys stellatus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00085	-	L-lysine	pH 7, 37°C, EDTA-treated protein	Platichthys stellatus
0.0302	-	L-lysine	pH 7, 37°C, untreated protein	Platichthys stellatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.00045	-	L-lysine	pH 7, 37°C, untreated protein	Platichthys stellatus
0.00085	-	L-lysine	pH 7, 37°C, EDTA-treated protein	Platichthys stellatus

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Release 2023.1 (January 2023)