Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | complete inhibition of isoform LAAOII and about 30% inhibition of isoform LAAOI at 5 mM | Cerastes cerastes | |
2-mercaptoethanol | complete inhibition of isoform LAAOII and about 50% inhibition of isoform LAAOI at 5 mM | Cerastes cerastes | |
Al3+ | strong inhibition of both isoforms at 10 mM | Cerastes cerastes | |
Co2+ | strong inhibition of both isoforms at 10 mM | Cerastes cerastes | |
Cu2+ | strong inhibition of both isoforms at 10 mM | Cerastes cerastes | |
EDTA | about 40% inhibition of isoform LAAOII and about 10% inhibition of isoform LAAOI at 5 mM | Cerastes cerastes | |
iodoacetic acid | about 45% inhibition of isoform LAAOII and no inhibition of isoform LAAOI at 5 mM | Cerastes cerastes | |
L-cysteine | complete inhibition of both isoforms at 5 mM | Cerastes cerastes | |
Ni2+ | strong inhibition of both isoforms at 10 mM | Cerastes cerastes | |
phenylmethylsulfonyl fluoride | about 20% inhibition of isoform LAAOII and about 90% inhibition of isoform LAAOI at 5 mM | Cerastes cerastes | |
reduced glutathione | complete inhibition of both isoforms at 5 mM | Cerastes cerastes | |
Urea | about 50% inhibition of isoform LAAOII and about 20% inhibition of isoform LAAOI at 5 mM | Cerastes cerastes | |
Zn2+ | strong inhibition of both isoforms at 10 mM | Cerastes cerastes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
L-Leu | isoform LAAOI, at pH 7.8 and 50°C | Cerastes cerastes | |
0.82 | - |
L-Leu | isoform LAAOII, at pH 7.0 and 60°C | Cerastes cerastes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 10 mM Mn2+ increases the activity of isoform LAAOII more than 2fold | Cerastes cerastes | |
additional information | Na+, K+, Ca2+, Mg2+ and Ba2+ ions show a non-significant increase in the enzymatic activity | Cerastes cerastes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
gel filtration | Cerastes cerastes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerastes cerastes | X2JCV5 | - |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sepharose column chromatography, and Superdex 200 gel filtration | Cerastes cerastes |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Cerastes cerastes | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Arg + H2O + O2 | best substrate | Cerastes cerastes | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-Ile + H2O + O2 | - |
Cerastes cerastes | 3-methyl-2-oxo-pentanoic acid + NH3 + H2O2 | - |
? | |
L-Leu + H2O + O2 | - |
Cerastes cerastes | 2-oxo-4-methylvaleric acid + NH3 + H2O2 | - |
? | |
L-Met + H2O + O2 | - |
Cerastes cerastes | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-Phe + H2O + O2 | - |
Cerastes cerastes | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-Trp + H2O + O2 | - |
Cerastes cerastes | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
L-Tyr + H2O + O2 | - |
Cerastes cerastes | 4-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
additional information | the enzyme isoforms show low affinity for L-Val, L-Ala, L-Asn, L-Gln and no specificity toward L-Pro, L-Ser, L-Thr, L-Cys, L-Gly and L-Asp | Cerastes cerastes | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 60000 + 1 * 53000, isoform LAAOII, SDS-PAGE | Cerastes cerastes |
heterodimer | 1 * 60000 + 1 * 56000, isoform LAAOI, SDS-PAGE | Cerastes cerastes |
Synonyms | Comment | Organism |
---|---|---|
LAAOI | isoform | Cerastes cerastes |
LAAOII | isoform | Cerastes cerastes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
isoform LAAOI | Cerastes cerastes |
60 | - |
isoform LAAOI | Cerastes cerastes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 70 | the enzyme isoforms are thermally stable up to 70°C for 1 h since no loss of enzymatic activities are recorded up to 40°C for isoform LAAOI and 50°C for LAAOII, whereas 50% loss of the enzymatic activities are recorded at 60°C for isoform LAAOI and 65°C for isoform LAAOII, respectively. On the other hand, a complete loss of activities is detected above 70°C for both isoforms | Cerastes cerastes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
isoform LAAOI | Cerastes cerastes |
7.8 | - |
isoform LAAOI | Cerastes cerastes |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | both enzyme isoform maintain their full activity at a wide range of pH from 4.0 to 9.0 for 1 h | Cerastes cerastes |