Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | enzymatic activity is stimulated 50-100fold by SDS treatment | Rhizoctonia solani |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 CodonPlus (DE)-RP and Arctic Express (DE) cells | Rhizoctonia solani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
L-Arg | at pH 8.5 and 30°C | Rhizoctonia solani | |
1.3 | - |
L-Lys | at pH 8.5 and 30°C | Rhizoctonia solani | |
5.6 | - |
L-Orn | at pH 8.5 and 30°C | Rhizoctonia solani | |
6.1 | - |
L-Leu | at pH 8.5 and 30°C | Rhizoctonia solani | |
6.8 | - |
L-Phe | at pH 8.5 and 30°C | Rhizoctonia solani | |
11.7 | - |
L-phenylalanine methyl ester | at pH 8.5 and 30°C | Rhizoctonia solani | |
20 | - |
L-Met | at pH 8.5 and 30°C | Rhizoctonia solani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizoctonia solani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
amylose column chromatography and Ni-NTA column chromatography | Rhizoctonia solani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-norleucine + H2O + O2 | 53% activity compared to L-Arg | Rhizoctonia solani | 2-oxocaproate + NH3 + H2O2 | - |
? | |
L-Arg + H2O + O2 | 100% activity | Rhizoctonia solani | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-Asp + H2O + O2 | 1.0% activity compared to L-Arg | Rhizoctonia solani | oxaloacetate + NH3 + H2O2 | - |
? | |
L-Glu + H2O + O2 | 1.6% activity compared to L-Arg | Rhizoctonia solani | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-His + H2O + O2 | 5.2% activity compared to L-Arg | Rhizoctonia solani | 2-oxo-4-imidazolepropionic acid + NH3 + H2O2 | - |
? | |
L-Ile + H2O + O2 | 3.1% activity compared to L-Arg | Rhizoctonia solani | 3-methyl-2-oxo-pentanoic acid + NH3 + H2O2 | - |
? | |
L-Leu + H2O + O2 | 70% activity compared to L-Arg | Rhizoctonia solani | 2-oxo-4-methylvaleric acid + NH3 + H2O2 | - |
? | |
L-leucine methyl ester + H2O + O2 | 61% activity compared to L-Arg | Rhizoctonia solani | methyl 2-oxo-4-methylpentanoate + NH3 + H2O2 | - |
? | |
L-Lys + H2O + O2 | 55% activity compared to L-Arg | Rhizoctonia solani | 6-amino-2-oxo-hexanoic acid + NH3 + H2O2 | - |
? | |
L-Met + H2O + O2 | 23% activity compared to L-Arg | Rhizoctonia solani | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-methionine methyl ester + H2O + O2 | 12% activity compared to L-Arg | Rhizoctonia solani | methyl 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-Orn + H2O + O2 | 60% activity compared to L-Arg | Rhizoctonia solani | 2-oxo-5-aminopentanoate + NH3 + H2O2 | - |
? | |
L-Phe + H2O + O2 | 88% activity compared to L-Arg | Rhizoctonia solani | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-phenylalanine methyl ester + H2O + O2 | 67% activity compared to L-Arg | Rhizoctonia solani | methyl phenylpyruvate + NH3 + H2O2 | - |
? | |
L-Trp + H2O + O2 | 6.4% activity compared to L-Arg | Rhizoctonia solani | indole-3-pyruvate + NH3 + H2O2 | - |
? | |
L-Tyr + H2O + O2 | 4.0% activity compared to L-Arg | Rhizoctonia solani | 4-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
additional information | N-methyl-L-phenylalanine, L-(alpha)-methylphenylalanine, N-acetyl-L-phenylalanine methyl ester, 2-phenylethylamine, N-alpha-acetyl-L-arginine, L-canavanine, L-serine, L-cysteine, L-threonine, D-phenylalanine, L-phenylglycine, (S)-beta-phenylalanine, L-proline, L-tert-leucine, L-valine, and L-alanine are no substrates | Rhizoctonia solani | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 65000, SDS-PAGE | Rhizoctonia solani |
Synonyms | Comment | Organism |
---|---|---|
L-AAO1 | - |
Rhizoctonia solani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
L-Met | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.09 | - |
L-Lys | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.1 | - |
L-Orn | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.13 | - |
L-Arg | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.13 | - |
L-Leu | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.16 | - |
L-Phe | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.16 | - |
L-phenylalanine methyl ester | at pH 8.5 and 30°C | Rhizoctonia solani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | - |
Rhizoctonia solani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhizoctonia solani |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
L-Met | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.01 | - |
L-phenylalanine methyl ester | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.02 | - |
L-Phe | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.02 | - |
L-Leu | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.02 | - |
L-Orn | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.07 | - |
L-Lys | at pH 8.5 and 30°C | Rhizoctonia solani | |
0.3 | - |
L-Arg | at pH 8.5 and 30°C | Rhizoctonia solani |