Literature summary for 1.4.3.2 extracted from

Vargas, L.J.; Quintana, J.C.; Pereanez, J.A.; Nunez, V.; Sanz, L.; Calvete, J.
Cloning and characterization of an antibacterial L-amino acid oxidase from Crotalus durissus cumanensis venom (2013), Toxicon, 64, 1-11.

Search for more literature for 1.4.3.2

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00923	-	L-leucine	in 50 mM Tris-HCl buffer pH 8.0, at 37°C	Crotalus durissus cumanensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	1 * 55000, SDS-PAGE	Crotalus durissus cumanensis
56827	-	1 * 56827, calculated from amino acid sequence	Crotalus durissus cumanensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-leucine + H2O + O2	Crotalus durissus cumanensis	-	4-methyl-2-oxopentanoic acid + NH3 + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Crotalus durissus cumanensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE column chromatography and Sephacryl-S200 gel filtration	Crotalus durissus cumanensis

Source Tissue

Source Tissue	Comment	Organism	Textmining
venom	-	Crotalus durissus cumanensis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-leucine + H2O + O2	-	Crotalus durissus cumanensis	4-methyl-2-oxopentanoic acid + NH3 + H2O2	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 55000, SDS-PAGE	Crotalus durissus cumanensis
monomer	1 * 56827, calculated from amino acid sequence	Crotalus durissus cumanensis

Synonyms

Synonyms	Comment	Organism
L-amino acid oxidase	-	Crotalus durissus cumanensis
LAAO	-	Crotalus durissus cumanensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.8	-	L-leucine	in 50 mM Tris-HCl buffer pH 8.0, at 37°C	Crotalus durissus cumanensis

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Crotalus durissus cumanensis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Crotalus durissus cumanensis	calculated from amino acid sequence	-	8

General Information

General Information	Comment	Organism
physiological function	the enzyme inhibits in a dose-dependent manner the growth of Staphylococcus aureus and Acinetobacter baumannii, however, against Escherichia coli the enzyme does not show inhibitory capacity and does not exhibit cytotoxic activity on the mouse myoblast cell line C2C12 and on peripheral blood mononuclear cell	Crotalus durissus cumanensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
195	-	L-leucine	in 50 mM Tris-HCl buffer pH 8.0, at 37°C	Crotalus durissus cumanensis

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Release 2023.1 (January 2023)