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Literature summary for 1.4.3.2 extracted from

  • Yang, C.A.; Cheng, C.H.; Liu, S.Y.; Lo, C.T.; Lee, J.W.; Peng, K.C.
    Identification of antibacterial mechanism of L-amino acid oxidase derived from Trichoderma harzianum ETS 323 (2011), FEBS J., 278, 3381-3394.
    View publication on PubMed

Application

Application Comment Organism
medicine the antibacterial activity of the enzyme is similar to that of kanamycin Trichoderma harzianum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.73
-
L-phenylalanine at pH 7.0 and 40°C Trichoderma harzianum
12.01
-
L-phenylalanine at pH 7.0 and 30°C Trichoderma harzianum
12.81
-
L-phenylalanine at pH 7.0 and 20°C Trichoderma harzianum
13.52
-
L-phenylalanine at pH 7.0 and 50°C Trichoderma harzianum

Organism

Organism UniProt Comment Textmining
Trichoderma harzianum
-
-
-
Trichoderma harzianum ETS 323
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trichoderma harzianum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + H2O + O2
-
Trichoderma harzianum phenylpyruvate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2
-
Trichoderma harzianum ETS 323 phenylpyruvate + NH3 + H2O2
-
?

Synonyms

Synonyms Comment Organism
L-amino acid oxidase
-
Trichoderma harzianum
LAAO
-
Trichoderma harzianum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
at pH 7.0 Trichoderma harzianum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 45 decrease of activity to 35-50% and 90-78% of maximal activity at 20-30°C and 45-50°C, respectively Trichoderma harzianum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
the enzyme rapidly becomes thermally denatured at temperatures above 40°C Trichoderma harzianum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
L-phenylalanine at pH 7.0 and 20°C Trichoderma harzianum
0.013
-
L-phenylalanine at pH 7.0 and 30°C Trichoderma harzianum
0.02
-
L-phenylalanine at pH 7.0 and 50°C Trichoderma harzianum
0.025
-
L-phenylalanine at pH 7.0 and 40°C Trichoderma harzianum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Trichoderma harzianum

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5
-
Trichoderma harzianum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme is stable at pH 6.0-8.0 (90% activity) and less stable at both pH 4.0-5.5 (less than 40% of maximal activity) and pH 9.0 (77% of maximal activity) Trichoderma harzianum

Cofactor

Cofactor Comment Organism Structure
FAD
-
Trichoderma harzianum

General Information

General Information Comment Organism
physiological function the enzyme interacts with bacteria to cause membrane permeabilization. Reactive oxidative species accumulation triggers forms of cell damage, including lipid peroxidation and DNA strand breakage that results in bacterial growth inhibition Trichoderma harzianum