Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells and in Pichia pastoris strain KM71H | Daboia russelii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
L-Tyr | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
0.142 | - |
L-Phe | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
0.318 | - |
L-Trp | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
0.373 | - |
L-Met | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
0.49 | - |
L-Leu | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
1.4 | - |
L-Ile | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
12.2 | - |
L-Arg | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
13.92 | - |
L-Val | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
64 | - |
L-Lys | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
116.5 | - |
L-Ala | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54864 | - |
x * 54864, calculated from amino acid sequence | Daboia russelii |
62025 | - |
x * 62025, MALDI-TOF mass spectrometry | Daboia russelii |
63600 | - |
x * 63600, SDS-PAGE | Daboia russelii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-amino acid + H2O + O2 | Daboia russelii | - |
a 2-oxo carboxylate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daboia russelii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme has three potential glycosylation sites at Asn 172, 194, and 361 | Daboia russelii |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Mono Q column chromatography, and heparin-Sepharose column chromatography | Daboia russelii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Daboia russelii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala + H2O + O2 | the substrate is not effectively oxidized | Daboia russelii | 2-oxopropanoate + NH3 + H2O2 | - |
? | |
L-amino acid + H2O + O2 | - |
Daboia russelii | a 2-oxo carboxylate + NH3 + H2O2 | - |
? | |
L-Arg + H2O + O2 | the substrate is not effectively oxidized | Daboia russelii | 5-carbamidamido-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-Ile + H2O + O2 | - |
Daboia russelii | 3-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-Leu + H2O + O2 | best substrate | Daboia russelii | 4-methyl-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-Lys + H2O + O2 | the substrate is not effectively oxidized | Daboia russelii | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-Met + H2O + O2 | - |
Daboia russelii | 4-methylthio-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-Phe + H2O + O2 | - |
Daboia russelii | 2-oxo-3-phenylpropanoate + NH3 + H2O2 | - |
? | |
L-Trp + H2O + O2 | - |
Daboia russelii | 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2 | - |
? | |
L-Tyr + H2O + O2 | - |
Daboia russelii | 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + H2O2 | - |
? | |
L-Val + H2O + O2 | the substrate is not effectively oxidized | Daboia russelii | 3-methyl-2-oxobutanoate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54864, calculated from amino acid sequence | Daboia russelii |
? | x * 62025, MALDI-TOF mass spectrometry | Daboia russelii |
? | x * 63600, SDS-PAGE | Daboia russelii |
Synonyms | Comment | Organism |
---|---|---|
L-amino acid oxidase | - |
Daboia russelii |
LAAO | - |
Daboia russelii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Daboia russelii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Daboia russelii | calculated from amino acid sequence | - |
8.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
L-Ala | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
0.03 | - |
L-Lys | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
0.11 | - |
L-Val | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
0.94 | - |
L-Arg | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
3.72 | - |
L-Ile | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
18.02 | - |
L-Trp | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
21.53 | - |
L-Leu | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
21.93 | - |
L-Met | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
40.56 | - |
L-Tyr | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii | |
41.3 | - |
L-Phe | pH 7.5, 0.9% NaCl, at 25°C | Daboia russelii |