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Literature summary for 1.4.3.2 extracted from

  • Chen, H.S.; Wang, Y.M.; Huang, W.T.; Huang, K.F.; Tsai, I.H.
    Cloning, characterization and mutagenesis of Russells viper venom L-amino acid oxidase: Insights into its catalytic mechanism (2012), Biochimie, 94, 335-344.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells and in Pichia pastoris strain KM71H Daboia russelii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
L-Tyr pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
0.142
-
L-Phe pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
0.318
-
L-Trp pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
0.373
-
L-Met pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
0.49
-
L-Leu pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4
-
L-Ile pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
12.2
-
L-Arg pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
13.92
-
L-Val pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
64
-
L-Lys pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
116.5
-
L-Ala pH 7.5, 0.9% NaCl, at 25°C Daboia russelii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54864
-
x * 54864, calculated from amino acid sequence Daboia russelii
62025
-
x * 62025, MALDI-TOF mass spectrometry Daboia russelii
63600
-
x * 63600, SDS-PAGE Daboia russelii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-amino acid + H2O + O2 Daboia russelii
-
a 2-oxo carboxylate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Daboia russelii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has three potential glycosylation sites at Asn 172, 194, and 361 Daboia russelii

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Mono Q column chromatography, and heparin-Sepharose column chromatography Daboia russelii

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Daboia russelii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Ala + H2O + O2 the substrate is not effectively oxidized Daboia russelii 2-oxopropanoate + NH3 + H2O2
-
?
L-amino acid + H2O + O2
-
Daboia russelii a 2-oxo carboxylate + NH3 + H2O2
-
?
L-Arg + H2O + O2 the substrate is not effectively oxidized Daboia russelii 5-carbamidamido-2-oxopentanoate + NH3 + H2O2
-
?
L-Ile + H2O + O2
-
Daboia russelii 3-methyl-2-oxopentanoate + NH3 + H2O2
-
?
L-Leu + H2O + O2 best substrate Daboia russelii 4-methyl-2-oxopentanoate + NH3 + H2O2
-
?
L-Lys + H2O + O2 the substrate is not effectively oxidized Daboia russelii 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-Met + H2O + O2
-
Daboia russelii 4-methylthio-2-oxobutanoate + NH3 + H2O2
-
?
L-Phe + H2O + O2
-
Daboia russelii 2-oxo-3-phenylpropanoate + NH3 + H2O2
-
?
L-Trp + H2O + O2
-
Daboia russelii 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
-
?
L-Tyr + H2O + O2
-
Daboia russelii 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + H2O2
-
?
L-Val + H2O + O2 the substrate is not effectively oxidized Daboia russelii 3-methyl-2-oxobutanoate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 54864, calculated from amino acid sequence Daboia russelii
? x * 62025, MALDI-TOF mass spectrometry Daboia russelii
? x * 63600, SDS-PAGE Daboia russelii

Synonyms

Synonyms Comment Organism
L-amino acid oxidase
-
Daboia russelii
LAAO
-
Daboia russelii

Cofactor

Cofactor Comment Organism Structure
FAD
-
Daboia russelii

pI Value

Organism Comment pI Value Maximum pI Value
Daboia russelii calculated from amino acid sequence
-
8.6

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.02
-
L-Ala pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
0.03
-
L-Lys pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
0.11
-
L-Val pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
0.94
-
L-Arg pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
3.72
-
L-Ile pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
18.02
-
L-Trp pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
21.53
-
L-Leu pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
21.93
-
L-Met pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
40.56
-
L-Tyr pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
41.3
-
L-Phe pH 7.5, 0.9% NaCl, at 25°C Daboia russelii