Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.2 extracted from

  • Sun, M.Z.; Guo, C.; Tian, Y.; Chen, D.; Greenaway, F.T.; Liu, S.
    Biochemical, functional and structural characterization of Akbu-LAAO: a novel snake venom L-amino acid oxidase from Agkistrodon blomhoffii ussurensis (2010), Biochimie, 92, 343-349.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Gd3+ inhibits 17% at 10 mM Gloydius ussuriensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
L-Leu pH 4.7, 37°C Gloydius ussuriensis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Gloydius ussuriensis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Gloydius ussuriensis
Ce3+ activates Gloydius ussuriensis
Co2+ activates Gloydius ussuriensis
Mg2+ activates Gloydius ussuriensis
Mn2+ activates Gloydius ussuriensis
Nd3+ activates Gloydius ussuriensis
Tb3+ activates Gloydius ussuriensis
Zn2+ Akbu-LAAO contains four Zn2þ per dimer, that are unessential for the hydrolytic activity of the enzyme. The apoenzyme shows 61% of maximal activity. The zinc ions probably help maintain the structural integrity of the enzyme Gloydius ussuriensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
2 * 65000, SDS-PAGE Gloydius ussuriensis
124400
-
gel filtration Gloydius ussuriensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-Leu + H2O + O2 Gloydius ussuriensis
-
2-oxo-4-methylvaleric acid + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Gloydius ussuriensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 11.2fold from venom by anion exchange chromatography and 3 steps of gel filtration, one of which is HPLC Gloydius ussuriensis

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Gloydius ussuriensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9
-
purified native enzyme, pH 4.7, 37°C Gloydius ussuriensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu + H2O + O2
-
Gloydius ussuriensis 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 65000, SDS-PAGE Gloydius ussuriensis

Synonyms

Synonyms Comment Organism
Akbu-LAAO
-
Gloydius ussuriensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gloydius ussuriensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
first optimum Gloydius ussuriensis
8.5
-
second optimum Gloydius ussuriensis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Gloydius ussuriensis

General Information

General Information Comment Organism
physiological function Akbu-LAAO shows apparent anti-aggregation effects on human and rabbit platelets and exhibits a strong bacteriostasis effect on Staphylococcus aureus, 18fold that of cephalosporin C Gloydius ussuriensis