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Literature summary for 1.4.3.2 extracted from

  • Stabeli, R.G.; SantAna, C.D.; Ribeiro, P.H.; Costa, T.R.; Ticli, F.K.; Pires, M.G.; Nomizo, A.; Albuquerque, S.; Malta-Neto, N.R.; Marins, M.; Sampaio, S.V.; Soares, A.M.
    Cytotoxic L-amino acid oxidase from Bothrops moojeni: biochemical and functional characterization (2007), Int. J. Biol. Macromol., 41, 132-140.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information the glycosylation site of the enzyme is not important for structural delineation of the active site Bothrops moojeni

Application

Application Comment Organism
medicine LAAO-I exhibited bactericidal, antitumoral, trypanocidal, edematogenic, and platelet-aggregating activities. It induces typical apoptotic DNA fragmentation in HL-60 cells. Potential use of LAAO-I as a therapeutic agent for treatment of diseases in which induction of H2O2 production can be beneficial Bothrops moojeni

Inhibitors

Inhibitors Comment Organism Structure
additional information is inactivated by freezing or low pH. Bactericidal, antitumoral, trypanocidal, edematogenic, platelet-aggregating activities, and apoptotic DNA fragmentation in HL-60 cells are inhibited by catalase Bothrops moojeni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64889
-
2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE Bothrops moojeni
66000
-
2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE Bothrops moojeni
130800
-
mass spectrometry Bothrops moojeni

Organism

Organism UniProt Comment Textmining
Bothrops moojeni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by ion-exchange chromatography and gel filtration, 12.9fold Bothrops moojeni

Source Tissue

Source Tissue Comment Organism Textmining
venom
-
Bothrops moojeni
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
with glycine or L-lysine as substrate Bothrops moojeni
10
-
with L-glutamine as substrate Bothrops moojeni
35
-
with L-serine as substrate Bothrops moojeni
37.8
-
-
Bothrops moojeni
40
-
with L-threonine or L-proline as substrate Bothrops moojeni
90
-
with L-arginine as substrate Bothrops moojeni
140
-
with L-histidine as substrate Bothrops moojeni
230
-
with L-alanine as substrate Bothrops moojeni
430
-
with L-valine as substrate Bothrops moojeni
435
-
with L-isoleucine as substrate Bothrops moojeni
460
-
with L-methionine as substrate Bothrops moojeni
480
-
with L-leucine as substrate Bothrops moojeni
487.7
-
12.9fold purified Bothrops moojeni
585
-
with L-tryptophan as substrate Bothrops moojeni
635
-
with L-tyrosine as substrate Bothrops moojeni
665
-
with L-phenylalanine as substrate Bothrops moojeni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycine + H2O + O2
-
Bothrops moojeni ? + NH3 + H2O2
-
?
L-alanine + H2O + O2
-
Bothrops moojeni 2-oxopropanoic acid + NH3 + H2O2
-
?
L-arginine + H2O + O2
-
Bothrops moojeni 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
?
L-glutamic acid + H2O + O2
-
Bothrops moojeni 2-oxoglutarate + NH3 + H2O2
-
?
L-histidine + H2O + O2
-
Bothrops moojeni 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
-
?
L-isoleucine + H2O + O2 high catalytic activity Bothrops moojeni 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-leucine + H2O + O2 high affinity for the substrate Bothrops moojeni 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
L-lysine + H2O + O2
-
Bothrops moojeni 6-amino-2-oxohexanoic acid + NH3 + H2O2
-
?
L-methionine + H2O + O2 high affinity for the substrate Bothrops moojeni 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
?
L-norleucine + H2O + O2 high catalytic activity Bothrops moojeni 2-oxohexanoate + NH3 + H2O2
-
?
L-phenylalanine + H2O + O2 high affinity for the substrate Bothrops moojeni phenylpyruvate + NH3 + H2O2
-
?
L-proline + H2O + O2
-
Bothrops moojeni ? + NH3 + H2O2
-
?
L-serine + H2O + O2
-
Bothrops moojeni 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
?
L-threonine + H2O + O2
-
Bothrops moojeni 2-oxo-3-hydroxybutanoic acid + NH3 + H2O2
-
?
L-tryptophan + H2O + O2 high affinity for the substrate Bothrops moojeni 3-indole-2-oxopropanoate + NH3 + H2O2
-
?
L-tyrosine + H2O + O2 high affinity for the substrate Bothrops moojeni p-hydroxyphenylpyruvate + NH3 + H2O2
-
?
L-valine + H2O + O2
-
Bothrops moojeni 2-oxoisovalerate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE Bothrops moojeni

Synonyms

Synonyms Comment Organism
LAAO-I
-
Bothrops moojeni

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
at temperatures lower than 4°C, enzymatic activity is completely lost Bothrops moojeni

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
at a pH below 4, enzymatic activity is completely lost Bothrops moojeni

pI Value

Organism Comment pI Value Maximum pI Value
Bothrops moojeni isoelectric focusing
-
4.7