Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the glycosylation site of the enzyme is not important for structural delineation of the active site | Bothrops moojeni |
Application | Comment | Organism |
---|---|---|
medicine | LAAO-I exhibited bactericidal, antitumoral, trypanocidal, edematogenic, and platelet-aggregating activities. It induces typical apoptotic DNA fragmentation in HL-60 cells. Potential use of LAAO-I as a therapeutic agent for treatment of diseases in which induction of H2O2 production can be beneficial | Bothrops moojeni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | is inactivated by freezing or low pH. Bactericidal, antitumoral, trypanocidal, edematogenic, platelet-aggregating activities, and apoptotic DNA fragmentation in HL-60 cells are inhibited by catalase | Bothrops moojeni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64889 | - |
2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE | Bothrops moojeni |
66000 | - |
2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE | Bothrops moojeni |
130800 | - |
mass spectrometry | Bothrops moojeni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bothrops moojeni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ion-exchange chromatography and gel filtration, 12.9fold | Bothrops moojeni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
venom | - |
Bothrops moojeni | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5 | - |
with glycine or L-lysine as substrate | Bothrops moojeni |
10 | - |
with L-glutamine as substrate | Bothrops moojeni |
35 | - |
with L-serine as substrate | Bothrops moojeni |
37.8 | - |
- |
Bothrops moojeni |
40 | - |
with L-threonine or L-proline as substrate | Bothrops moojeni |
90 | - |
with L-arginine as substrate | Bothrops moojeni |
140 | - |
with L-histidine as substrate | Bothrops moojeni |
230 | - |
with L-alanine as substrate | Bothrops moojeni |
430 | - |
with L-valine as substrate | Bothrops moojeni |
435 | - |
with L-isoleucine as substrate | Bothrops moojeni |
460 | - |
with L-methionine as substrate | Bothrops moojeni |
480 | - |
with L-leucine as substrate | Bothrops moojeni |
487.7 | - |
12.9fold purified | Bothrops moojeni |
585 | - |
with L-tryptophan as substrate | Bothrops moojeni |
635 | - |
with L-tyrosine as substrate | Bothrops moojeni |
665 | - |
with L-phenylalanine as substrate | Bothrops moojeni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + H2O + O2 | - |
Bothrops moojeni | ? + NH3 + H2O2 | - |
? | |
L-alanine + H2O + O2 | - |
Bothrops moojeni | 2-oxopropanoic acid + NH3 + H2O2 | - |
? | |
L-arginine + H2O + O2 | - |
Bothrops moojeni | 2-oxo-5-guanidinovaleric acid + NH3 + H2O2 | - |
? | |
L-glutamic acid + H2O + O2 | - |
Bothrops moojeni | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
L-histidine + H2O + O2 | - |
Bothrops moojeni | 3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2 | - |
? | |
L-isoleucine + H2O + O2 | high catalytic activity | Bothrops moojeni | 3-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-leucine + H2O + O2 | high affinity for the substrate | Bothrops moojeni | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-lysine + H2O + O2 | - |
Bothrops moojeni | 6-amino-2-oxohexanoic acid + NH3 + H2O2 | - |
? | |
L-methionine + H2O + O2 | high affinity for the substrate | Bothrops moojeni | 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2 | - |
? | |
L-norleucine + H2O + O2 | high catalytic activity | Bothrops moojeni | 2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-phenylalanine + H2O + O2 | high affinity for the substrate | Bothrops moojeni | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-proline + H2O + O2 | - |
Bothrops moojeni | ? + NH3 + H2O2 | - |
? | |
L-serine + H2O + O2 | - |
Bothrops moojeni | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
L-threonine + H2O + O2 | - |
Bothrops moojeni | 2-oxo-3-hydroxybutanoic acid + NH3 + H2O2 | - |
? | |
L-tryptophan + H2O + O2 | high affinity for the substrate | Bothrops moojeni | 3-indole-2-oxopropanoate + NH3 + H2O2 | - |
? | |
L-tyrosine + H2O + O2 | high affinity for the substrate | Bothrops moojeni | p-hydroxyphenylpyruvate + NH3 + H2O2 | - |
? | |
L-valine + H2O + O2 | - |
Bothrops moojeni | 2-oxoisovalerate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 64889, mass spectrometry, 1 * 66000, SDS-PAGE | Bothrops moojeni |
Synonyms | Comment | Organism |
---|---|---|
LAAO-I | - |
Bothrops moojeni |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
at temperatures lower than 4°C, enzymatic activity is completely lost | Bothrops moojeni |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
at a pH below 4, enzymatic activity is completely lost | Bothrops moojeni |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bothrops moojeni | isoelectric focusing | - |
4.7 |